Structure of PDB 3phe Chain A

Receptor sequence

>3pheA (length=558)

SMSYTWTGALITPCAAEESKLPINALSNSLLRHHNMVYATTSRSAGLRQK
KVTFDRLQVLDDHYRDVLKEMKAKASTVKAKLLSVEEACKLTPPHSAKSK
FGYGAKDVRNLSSKAVNHIHSVWKDLLEDTVTPIDTTIMAKNEVFCVQRK
PARLIVFPDLGVRVCEKMALYDVVSTLPQVVMGSSYGFQYSPGQRVEFLV
NTWKSKKNPMGFSYDTRCFDSTVTENDIRVEESIYQCCDLAPEARQAIKS
LTERLYIGGPLTNSKGQNCGYRRCRASGVLTTSCGNTLTCYLKASAACRA
AKLQDCTMLVNGDDLVVICESAGTQEDAASLRVFTEAMTRYSAPPGDPPQ
PEYDLELITSCSSNVSVAHDASGKRVYYLTRDPTTPLARAAWETARHTPV
NSWLGNIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIYGACYSIE
PLDLPQIIERLHGLSAFSLHSYSPGEINRVASCLRKLGVPPLRVWRHRAR
SVRARLLSQGGRAATCGKYLFNWAVKTKLKLTPIPAASQLDLSGWFVAGY
SGGDIYHS

3D structure

• PDB: 3phe Quinolones as HCV NS5B polymerase inhibitors.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	C9A	A	L419 R422 M423 H475 S476 Y477 P479 I482 A486 L489 P496 L497 W528	L414 R417 M418 H470 S471 Y472 P474 I477 A481 L484 P491 L492 W523	MOAD: ic50=0.016uM PDBbind-CN: -logKd/Ki=7.80,IC50=16nM

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0008236 serine-type peptidase activity
• GO:0016887 ATP hydrolysis activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0039654 fusion of virus membrane with host endosome membrane
• GO:0039694 viral RNA genome replication
• GO:0046718 symbiont entry into host cell

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:3phe, PDBe:3phe, PDBj:3phe
• PDBsum: 3phe
• PubMed: 21145235
• UniProt: D0PY27