Structure of PDB 3pgu Chain A

Receptor sequence

>3pguA (length=408) Species: 83333 (Escherichia coli K-12)

AGEQLNEFSSSGLGRAYSGEGAIADDAGNVSRNPALITMFDRPTFSAGAV
YIDPDVNISGTSPSGRSLKADNIAPTAWVPNMHFVAPINDQFGWGASITS
NYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYRLNNAWSFGLGFNAVY
ARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDSNTKIAHL
NGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGRAFNNYGLQSGYL
TLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATSTSGDTLFQK
HEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNF
NYAFHHHH

3D structure

• PDB: 3pgu From the Cover: Ligand-gated diffusion across the bacterial outer membrane.
• Chain: A
• Resolution: 1.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	C8E	A	Y227 W275	Y227 W258
BS02	C8E	A	A96 S97 N129 L130	A96 S97 N129 L130
BS03	C8E	A	L369 Y389	L352 Y372
BS04	C8E	A	W411 F413	W394 F396
BS05	C8E	A	Q180 G181	Q180 G181
BS06	C8E	A	L128 F146 W209	L128 F146 W209
BS07	C8E	A	W287 T331 Y333 F341 R342	W270 T314 Y316 F324 R325
BS08	C8E	A	G132 F142 L144	G132 F142 L144
BS09	C8E	A	R282 Q286 Y335 D336	R265 Q269 Y318 D319
BS10	C8E	A	A167 Q169 N189	A167 Q169 N189

Gene Ontology

Molecular Function

• GO:0015483 long-chain fatty acid transporting porin activity
• GO:0022834 ligand-gated channel activity

Biological Process

• GO:0006869 lipid transport
• GO:0015909 long-chain fatty acid transport
• GO:0055085 transmembrane transport

Cellular Component

• GO:0009279 cell outer membrane

External links

• PDB: RCSB:3pgu, PDBe:3pgu, PDBj:3pgu
• PDBsum: 3pgu
• PubMed: 21593406
• UniProt: P10384|FADL_ECOLI Long-chain fatty acid transport protein (Gene Name=fadL)