Structure of PDB 3p7o Chain A

Receptor sequence
>3p7oA (length=958) Species: 10116 (Rattus norvegicus) [Search protein sequence]
MNNPAIQRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALD
VHIGSLSDPPNIPGLSHFCFHMLFLGTKKYPKENEYSQFLSEHAGSSNAF
TSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDASCKDREVNAVDSEHE
KNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVREEL
LKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEH
PFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHE
GPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDII
LHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKL
HYYPLNGVLTAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTDR
TEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTKNEFIPTNFEILALE
KDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCN
MAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPIL
LKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMT
EVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGV
MQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQRRNEVHNNCGI
EIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRAN
GIQGLRFIIQSEKPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAI
RRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLSKDDIIKFYK
EMLAVDAPRRHKVSVHVLAREMDSINLSEAPPLPQPEVIHNMTEFKRGLP
LFPLVKPH
3D structure
PDB3p7o Identification of the allosteric regulatory site of insulysin.
ChainA
Resolution2.1423 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.4.24.56: insulysin.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 peptide A H108 F111 F115 N139 A140 F141 E189 N196 A198 W199 R824 Y831 H67 F70 F74 N98 A99 F100 E148 N155 A157 W158 R783 Y790
BS02 peptide A H332 G339 E341 L359 V360 G361 Y609 H291 G298 E300 L318 V319 G320 Y568
Gene Ontology
Molecular Function
GO:0001540 amyloid-beta binding
GO:0004175 endopeptidase activity
GO:0004222 metalloendopeptidase activity
GO:0005524 ATP binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0008270 zinc ion binding
GO:0016887 ATP hydrolysis activity
GO:0017046 peptide hormone binding
GO:0031626 beta-endorphin binding
GO:0042277 peptide binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0043559 insulin binding
GO:0044877 protein-containing complex binding
GO:0046872 metal ion binding
Biological Process
GO:0006508 proteolysis
GO:0006979 response to oxidative stress
GO:0010815 bradykinin catabolic process
GO:0010992 ubiquitin recycling
GO:0019885 antigen processing and presentation of endogenous peptide antigen via MHC class I
GO:0030163 protein catabolic process
GO:0038020 insulin receptor recycling
GO:0042447 hormone catabolic process
GO:0043171 peptide catabolic process
GO:0045861 negative regulation of proteolysis
GO:0050435 amyloid-beta metabolic process
GO:0051603 proteolysis involved in protein catabolic process
GO:0097242 amyloid-beta clearance
GO:0150094 amyloid-beta clearance by cellular catabolic process
GO:1901142 insulin metabolic process
GO:1901143 insulin catabolic process
GO:1903715 regulation of aerobic respiration
Cellular Component
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005777 peroxisome
GO:0005782 peroxisomal matrix
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0009897 external side of plasma membrane
GO:0009986 cell surface
GO:0016323 basolateral plasma membrane
GO:0031597 cytosolic proteasome complex
GO:0031904 endosome lumen
GO:0070062 extracellular exosome

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3p7o, PDBe:3p7o, PDBj:3p7o
PDBsum3p7o
PubMed21731629
UniProtP35559|IDE_RAT Insulin-degrading enzyme (Gene Name=Ide)

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