Structure of PDB 3osp Chain A

Receptor sequence

>3ospA (length=428) Species: 4932 (Saccharomyces cerevisiae)

KRIVACDDPDFLTSYFAHSRLHHLSAWKANLKDKFLNENIHKYTKITDKD
TYIIFHIDFDCFFATVAYLCRSSSFSACDFKRDPIVVCHGTKNSDIASCN
YVARSYGIKNGMWVSQAEKMLPNGIKLISLPYTFEQFQLKSEAFYSTLKR
LNIFNLILPISIDEAVCVRIIPDTLNARLCEEIRQEIFQGTNGCTVSIGC
SDSLVLARLALKMAKPNGYNITFKSNLSEEFWSSFKLDDLPGVGHSTLSR
LESTFDSPHSLNDLRKRYTLDALKASVGSKLGMKIHLALQGQDDEESLKI
LYDPKEVLQRKSLSIDINWGIRFKNITQVDLFIERGCQYLLEKLNEINKT
TSQITLKLMRRCKDAPIEPPKYMGMGRCDSFSRSSRLGIPTNEFGIIATE
MKSLYRTLGCPPMELRGLALQFNKLVDV

3D structure

• PDB: 3osp DNA synthesis across an abasic lesion by yeast REV1 DNA polymerase.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	I307 Y319 F320 H322 S323 R324 L325 H326 S329 K336 H393 G394 T395 K396 N397 S398 D399 K590 E606 K621 S624 I625 D626 N628 W629 K681 Y682	I3 Y15 F16 H18 S19 R20 L21 H22 S25 K32 H89 G90 T91 K92 N93 S94 D95 K280 E296 K311 S314 I315 D316 N318 W319 K371 Y372
BS02	dna	A	P551 G552 G554 H555 S556 T557 S692 S694 R696	P241 G242 G244 H245 S246 T247 S382 S384 R386
BS03	DCP	A	R324 D362 F363 D364 C365 F366 F367 A401 S402 Y405 R408 N414 D467 K525	R20 D58 F59 D60 C61 F62 F63 A97 S98 Y101 R104 N110 D163 K215
BS04	MG	A	D362 D467 E468	D58 D163 E164
BS05	MG	A	D362 F363 D467	D58 F59 D163

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3osp, PDBe:3osp, PDBj:3osp
• PDBsum: 3osp
• PubMed: 21167175
• UniProt: P12689|REV1_YEAST DNA repair protein REV1 (Gene Name=REV1)