Structure of PDB 3one Chain A

Receptor sequence

>3oneA (length=487) Species: 3873 (Lupinus luteus)

SHMALLVEKTTSGREYKVKDMSQADFGRLEIELAEVEMPGLMASRSEFGP
SQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDHAAAA
IARDSAAVFAWKGETLQEYWWCTERALDWGPGGGPDLIVDDGGDTTLLIH
EGVKAEEIYEKSGQFPDPDSTDNAEFKIVLSIIKEGLKTDPKRYHKMKDR
VVGVSEETTTGVKRLYQMQANGTLLFPAINVNDSVTKSKFDNLYGCRHSL
PDGLMRATDVMIAGKVAVVAGYGDVGKGCAAALKQAGARVIVTEIDPICA
LQATMEGLQVLTLEDVVSEADIFVTTTGNKDIIMLDHMKKMKNNAIVCNI
GHFDNEIDMLGLETHPGVKRITIKPQTDRWVFPETNTGIIILAEGRLMNL
GCATGHPSFVMSCSFTNQVIAQLELWNEKSSGKYEKKVYVLPKHLDEKVA
ALHLEKLGAKLTKLSKDQADYISVPVEGPYKPFHYRY

3D structure

• PDB: 3one High-resolution structures of complexes of plant S-adenosyl-L-homocysteine hydrolase (Lupinus luteus).
• Chain: A
• Resolution: 1.35 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H62 S85 S90 D139 E205 N230 K235 D239 N240 C244 H350 H404 S412 Q416
• Catalytic site (residue number reindexed from 1): H64 S87 S92 D141 E207 N232 K237 D241 N242 C246 H352 H406 S414 Q418
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	T206 T207 T208 N240 G271 D272 V273 E292 I293 T325 N327 I330 I348 G349 H350 N397	T208 T209 T210 N242 G273 D274 V275 E294 I295 T327 N329 I332 I350 G351 H352 N399
BS02	ADE	A	T64 H404 M409	T66 H406 M411
BS03	NA	A	Q66 T402 G403 H404 P405	Q68 T404 G405 H406 P407
BS04	NAD	A	K479 Y483	K481 Y485

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:3one, PDBe:3one, PDBj:3one
• PDBsum: 3one
• PubMed: 22349223
• UniProt: Q9SP37|SAHH_LUPLU Adenosylhomocysteinase (Gene Name=SAHH)