Structure of PDB 3ole Chain A

Receptor sequence
>3oleA (length=495) Species: 9606 (Homo sapiens) [Search protein sequence]
YSPNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVAI
YNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVINH
MCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIENY
NDATQVRDCRLTGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKH
MWPGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNG
RVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDN
QRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNG
NDVNDWVGPPNNNGVIKEVTINPDTTCGNDWVCEHRWRQIRNMVIFRNVV
DGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWSFSLTLQTGLPAGTYCD
VISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL
3D structure
PDB3ole Structures of human pancreatic alpha-amylase in complex with acarviostatins: Implications for drug design against type II diabetes.
ChainA
Resolution1.55 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D197 S226 D300
Catalytic site (residue number reindexed from 1) D196 S225 D299
Enzyme Commision number 3.2.1.1: alpha-amylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A W58 W59 Q63 H305 W57 W58 Q62 H304 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS02 G6D A W59 Q63 T163 W58 Q62 T162 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS03 GLC A Y151 K200 E240 Y150 K199 E239 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS04 G6D A H201 E233 G306 H200 E232 G305 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS05 GLC A W134 K172 Y174 W133 K171 Y173 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS06 GLC A S132 W134 D135 Y174 S131 W133 D134 Y173 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS07 GLC A K322 T377 W388 Q390 K321 T376 W387 Q389 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS08 GLC A R387 R389 Q390 R386 R388 Q389 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS09 CA A N100 R158 D167 H201 N99 R157 D166 H200
BS10 ACI A W58 Y62 H101 D197 E233 H299 D300 W57 Y61 H100 D196 E232 H298 D299 PDBbind-CN: -logKd/Ki=7.83,Ki=14.7nM
BS11 PCA A Y2 I230 N250 Y1 I229 N249
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004556 alpha-amylase activity
GO:0005509 calcium ion binding
GO:0016160 amylase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0031404 chloride ion binding
GO:0043169 cation binding
GO:0046872 metal ion binding
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0016052 carbohydrate catabolic process
GO:0044245 polysaccharide digestion
Cellular Component
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0070062 extracellular exosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3ole, PDBe:3ole, PDBj:3ole
PDBsum3ole
PubMed21111049
UniProtP04746|AMYP_HUMAN Pancreatic alpha-amylase (Gene Name=AMY2A)

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