Structure of PDB 3ola Chain A

Receptor sequence

>3olaA (length=461) Species: 12080 (Poliovirus 1)

GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRL
KTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDA
MYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQKLLDTYGI
NLPLVTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHK
NPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALK
MVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDY
GLTMTPADKSATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHES
IRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRALDLPEY
STLYRRWLDSF

3D structure

• PDB: 3ola Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase.
• Chain: A
• Resolution: 2.55 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P20 K24 E108 T114 S115 K127 Y157 I176 A178 R188 H199 G211 C212 D213 S288 G289 C290 L419	P20 K24 E108 T114 S115 K127 Y157 I176 A178 R188 H199 G211 C212 D213 S288 G289 C290 L419
BS02	rna	A	K133 D238 Y326 D328 K375 M392 S400 N409 H413 L420	K133 D238 Y326 D328 K375 M392 S400 N409 H413 L420
BS03	rna	A	I16 N18 G124	I16 N18 G124
BS04	ZN	A	H270 H272 C281	H270 H272 C281

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:3ola, PDBe:3ola, PDBj:3ola
• PDBsum: 3ola
• PubMed: 21148772
• UniProt: B3VQP5