Structure of PDB 3ol7 Chain A

Receptor sequence

>3ol7A (length=461) Species: 12080 (Poliovirus 1)

GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRL
KTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDA
MYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQKLLDTYGI
NLPLVTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHK
NPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALK
MVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDY
GLTMTPADKSATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHES
IRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRALDLPEY
STLYRRWLDSF

3D structure

• PDB: 3ol7 Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase.
• Chain: A
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	A19 P20 E108 T114 S115 K127 Y157 K159 I176 A178 R188 H199 G211 C212 D213 G289 C290 L419	A19 P20 E108 T114 S115 K127 Y157 K159 I176 A178 R188 H199 G211 C212 D213 G289 C290 L419
BS02	rna	A	K133 K159 R174 D238 S288 S294 Y326 D328 K375 S400 N409 H413	K133 K159 R174 D238 S288 S294 Y326 D328 K375 S400 N409 H413
BS03	MG	A	Y234 D328	Y234 D328
BS04	MG	A	D233 D328 D329	D233 D328 D329
BS05	POP	A	K167 G236	K167 G236

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:3ol7, PDBe:3ol7, PDBj:3ol7
• PDBsum: 3ol7
• PubMed: 21148772
• UniProt: P03300|POLG_POL1M Genome polyprotein