Structure of PDB 3ojo Chain A

Receptor sequence

>3ojoA (length=409) Species: 1280 (Staphylococcus aureus)

SKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQNGQISIEEPG
LQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRA
LDSILPFLKKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCP
ERVLPGKILEELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTA
EMSKLMENTYRDVNIALANELTKICNNLNINVLDVIEMANKHPRVNIHQP
GPGVGGHCLAVDPNAKLIQTGREINNSMPAYVVDTTKQIIKALSGNKVTV
FGLTYKGDVDDIRESPAFDIYELLNQEPDIEVCAYDPHVELDFVEHDMSH
AVKDASLVLILSDHSEFKNLSDSHFDKMKHKVIFDTKNVVKSSFEDVLYY
NYGNIFNFI

3D structure

• PDB: 3ojo Structure Analysis of the Staphylococcus aureus UDP-N-acetyl-mannosamine Dehydrogenase Cap5O Involved in Capsular Polysaccharide Biosynthesis.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T119 P155 K204 N208 C258 D262
• Catalytic site (residue number reindexed from 1): T119 P155 K204 N208 C258 D262

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	V6 G7 G9 Y10 I11 D30 I31 V80 P81 T82 T119 H257 R322	V6 G7 G9 Y10 I11 D30 I31 V80 P81 T82 T119 H257 R313
BS02	PDC	A	R211 Y314 K315	R211 Y305 K306
BS03	PDC	A	R152 K315	R152 K306
BS04	PDC	A	R152 E207 R211	R152 E207 R211
BS05	PDC	A	H242 R244	H242 R244

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
• GO:0051287 NAD binding

Biological Process

• GO:0000271 polysaccharide biosynthetic process

External links

• PDB: RCSB:3ojo, PDBe:3ojo, PDBj:3ojo
• PDBsum: 3ojo
• PubMed: 21454499
• UniProt: P95708