Structure of PDB 3oha Chain A

Receptor sequence

>3ohaA (length=516) Species: 4932 (Saccharomyces cerevisiae)

GDPHMSKFTWKELIQLGSPSKAYESSLACIAHIDMNAFFAQVEQMRCGLS
KEDPVVCVQWNSIIAVSYAARKYGISRMDTIQEALKKCSNLIPIHTAVFK
KGEDFWQYHDGCGSWVQDPAKQISVEDHKVSLEPYRRESRKALAIFKWAC
DLVERASIDEVFLDLGRICFNMLMFDNEYELTGDLKLKDALSNIREAFIG
GNYDINSHLPLIPEKIKSLKFEGDVFNPEGRDLITDWDDVILALGSQVCK
GIRDSIKDILGYTTSCGLSSTKNVCKLASNYKKPDAQTIVKNDCLLDFLD
CGKFEITSFWTLGGVLGKELIDVLDLPHENSIKHIRETWPDNAGQLKEFL
DAKVKQSDYDRSTSNIDPLKTADLAEKLFKLSRGRYGLPLSSRPVVKSMM
SNKNLRGKSCNSIVDCISWLEVFCAELTSRIQDLEQEYNKIVIPRTVSIS
LKTKSYEVYRKSGPVAYKGINFQSHELLKVGIKFVTDLDIKGKNKSYYPL
TKLSMTITNFDIIDLQ

3D structure

• PDB: 3oha Structural basis for error-free replication of oxidatively damaged DNA by yeast DNA polymerase eta.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E156 K272 W306 T307 G309 G310 V311 L312 R456 K457 S458 G459	E160 K276 W310 T311 G313 G314 V315 L316 R460 K461 S462 G463
BS02	dna	A	Q55 W56 R73 M74 K125 V126 R132 R389 S394 M395 M396 N398 N400 R426	Q59 W60 R77 M78 K129 V130 R136 R393 S398 M399 M400 N402 N404 R430
BS03	DCP	A	D30 M31 N32 A33 F34 F35 Y64 R73 D155 K279	D34 M35 N36 A37 F38 F39 Y68 R77 D159 K283
BS04	MG	A	D30 M31 D155	D34 M35 D159
BS05	MG	A	D30 E156	D34 E160

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3oha, PDBe:3oha, PDBj:3oha
• PDBsum: 3oha
• PubMed: 21070945
• UniProt: Q04049|POLH_YEAST DNA polymerase eta (Gene Name=RAD30)