Structure of PDB 3oh6 Chain A

Receptor sequence
>3oh6A (length=288) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MADIGSEFYTLNWQPPYDWSWMLGFLAARAVSSVETVADSYYARSLAVGE
YRGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNG
ALGRLGAARPGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYG
ERLDDFPEYICFPTPQRLAAADPQALKALGMPLKRAEALIHLANAALEGT
LPMTIPGDVEQAMKTLQTFPGIGRWTANYFALRGWQAKDVFLPDDCLIKQ
RFPGMTPAQIRRYAERWKPWRSYALLHIWYTEGWQPDE
3D structure
PDB3oh6 Structure of Escherichia coli AlkA in Complex with Undamaged DNA.
ChainA
Resolution2.894 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) W218 Y222 D238
Catalytic site (residue number reindexed from 1) W225 Y229 D245
Enzyme Commision number 3.2.2.21: DNA-3-methyladenine glycosylase II.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A L125 P213 G214 G216 W218 T219 L132 P220 G221 G223 W225 T226
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003905 alkylbase DNA N-glycosylase activity
GO:0005515 protein binding
GO:0008725 DNA-3-methyladenine glycosylase activity
GO:0016787 hydrolase activity
GO:0032131 alkylated DNA binding
GO:0043916 DNA-7-methylguanine glycosylase activity
GO:0052821 DNA-7-methyladenine glycosylase activity
GO:0052822 DNA-3-methylguanine glycosylase activity
Biological Process
GO:0006281 DNA repair
GO:0006284 base-excision repair
GO:0006285 base-excision repair, AP site formation
GO:0006307 DNA alkylation repair
GO:0006950 response to stress
GO:0006974 DNA damage response
Cellular Component
GO:0005737 cytoplasm
GO:0032993 protein-DNA complex

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Cellular Component
External links
PDB RCSB:3oh6, PDBe:3oh6, PDBj:3oh6
PDBsum3oh6
PubMed20843803
UniProtP04395|3MG2_ECOLI DNA-3-methyladenine glycosylase 2 (Gene Name=alkA)

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