Structure of PDB 3o8l Chain A

Receptor sequence

>3o8lA (length=748) Species: 9986 (Oryctolagus cuniculus)

ARTLGVGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGL
VDGGDHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKR
GITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITAEEATRSSYLNI
VGLVGSIDNDFCGTDMTIGTDSALHRITEIVDAITTTAQSHQRTFVLEVM
GRHCGYLALVTSLSCGADWVFIPECPPDDNWEDHLCRRLSETRTRGSRLN
IIIVAEGAIDRNGKPITSEGVKDLVVRRLGYDTRVTVLGHVQRGGTPSAF
DRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDV
TKAMDEKRFDEAMKLRGRSFMNNWEVYKLLAHIRPPAPKSGSYTVAVMNV
GAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGPAKGQIEEAGWSYVGG
WTGQGGSKLGSKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLEL
MEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQ
SAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQAN
VEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNV
LGHMQQGGSPTPFDRNFATKMGAKAMNWMAGKIKESYRNGRIFANTPDSG
CVLGMRKRALVFQPVTELQNQTDFEHRIPKEQWWLKLRPILKILAKYE

3D structure

• PDB: 3o8l The Crystal Structures of Eukaryotic Phosphofructokinases from Baker's Yeast and Rabbit Skeletal Muscle.
• Chain: A
• Resolution: 3.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G25 R88 D119 S164 D166 D168 R210
• Catalytic site (residue number reindexed from 1): G17 R80 D111 S156 D158 D160 R202
• Enzyme Commision number: 2.7.1.11: 6-phosphofructokinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	G24 Y55 R88 C89 R93 G118 G120 S121 T123 G124	G16 Y47 R80 C81 R85 G110 G112 S113 T115 G116
BS02	ADP	A	D173 M174 F308 F538 F671	D165 M166 F300 F530 F663
BS03	ATP	A	W227 H242 R246 Y385 H390 I391	W219 H234 R238 Y377 H382 I383
BS04	PO4	A	R424 G457 G458	R416 G449 G450
BS05	PO4	A	R471 S530 N532 R735	R463 S522 N524 R727

Gene Ontology

Molecular Function

• GO:0003872 6-phosphofructokinase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016208 AMP binding
• GO:0016301 kinase activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding
• GO:0048029 monosaccharide binding
• GO:0070095 fructose-6-phosphate binding

Biological Process

• GO:0006002 fructose 6-phosphate metabolic process
• GO:0006096 glycolytic process
• GO:0016310 phosphorylation
• GO:0030388 fructose 1,6-bisphosphate metabolic process
• GO:0061621 canonical glycolysis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005945 6-phosphofructokinase complex
• GO:0016020 membrane

External links

• PDB: RCSB:3o8l, PDBe:3o8l, PDBj:3o8l
• PDBsum: 3o8l
• PubMed: 21241708
• UniProt: P00511|PFKAM_RABIT ATP-dependent 6-phosphofructokinase, muscle type (Gene Name=PFKM)