Structure of PDB 3o7u Chain A

Receptor sequence
>3o7uA (length=422) Species: 562 (Escherichia coli) [Search protein sequence]
ALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLV
IPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQR
AWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVA
FPQEGILSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFAL
AQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSY
NGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVKEMLE
SGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLI
THHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKV
IASTQPAQTTVYLEQPEAIDYK
3D structure
PDB3o7u Three-dimensional structure and catalytic mechanism of Cytosine deaminase.
ChainA
Resolution1.708 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H61 H63 Q156 H214 E217 D313
Catalytic site (residue number reindexed from 1) H58 H60 Q153 H211 E214 D310
Enzyme Commision number 3.5.4.-
3.5.4.1: cytosine deaminase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PXN A W105 Q106 K109 W102 Q103 K106
BS02 ZN A H61 H63 H214 H58 H60 H211
BS03 ZN A H97 E138 H94 E135
BS04 O7U A H63 L81 Q156 H214 E217 H246 D313 D314 W319 H60 L78 Q153 H211 E214 H243 D310 D311 W316 MOAD: Ki=52nM
PDBbind-CN: -logKd/Ki=7.28,Ki=52nM
Gene Ontology
Molecular Function
GO:0004131 cytosine deaminase activity
GO:0008198 ferrous iron binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
GO:0035888 isoguanine deaminase activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding
Biological Process
GO:0006209 cytosine catabolic process
GO:0019858 cytosine metabolic process
Cellular Component
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:3o7u, PDBe:3o7u, PDBj:3o7u
PDBsum3o7u
PubMed21545144
UniProtP25524|CODA_ECOLI Cytosine deaminase (Gene Name=codA)

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