Structure of PDB 3o3r Chain A

Receptor sequence
>3o3rA (length=315) Species: 10116 (Rattus norvegicus) [Search protein sequence]
TTFVKLRTKAKMPLVGLGTWKSPPGQVKEAVKAAIDAGYRHFDCAYVYQN
ESEVGEAIQEKIKEKAVRREDLFIVSKLWSTFFEKSLMKEAFQKTLSDLK
LDYLDLYLIHWPQGLQAGKEFLPKDSQGKVLMSKSTFLDAWEGMEELVDQ
GLVKALGVSNFNHFQIERLLNKPGLKHKPVTNQVECHPYLTQEKLIQYCH
SKGIAVIAYSPLGSPDRPYAKPEDPVVLEIPKIKEIAAKHKKTIAQVLIR
FHVQRNVAVIPKSVTLSHIKENIQVFDFQLSEEDMAAILSLNRNWRACGL
FVTSDEEDFPFHEEY
3D structure
PDB3o3r Structure of rat aldose reductase-like protein AKR1B14 holoenzyme: Probing the role of His269 in coenzyme binding by site-directed mutagenesis
ChainA
Resolution1.86 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D44 Y49 K78 H111
Catalytic site (residue number reindexed from 1) D43 Y48 K77 H110
Enzyme Commision number 1.1.1.21: aldose reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAP A G19 T20 W21 D44 Y49 H111 S160 N161 Q184 Y210 S211 P212 L213 S215 P216 D217 A246 I261 P262 K263 S264 V265 T266 H269 E272 N273 G18 T19 W20 D43 Y48 H110 S159 N160 Q183 Y209 S210 P211 L212 S214 P215 D216 A245 I260 P261 K262 S263 V264 T265 H268 E271 N272
Gene Ontology
Molecular Function
GO:0004032 aldose reductase (NADPH) activity
GO:0004033 aldo-keto reductase (NADPH) activity
GO:0016491 oxidoreductase activity
GO:0036130 prostaglandin H2 endoperoxidase reductase activity
Biological Process
GO:0006693 prostaglandin metabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:3o3r, PDBe:3o3r, PDBj:3o3r
PDBsum3o3r
PubMed21168333
UniProtQ5RJP0|ALD1_RAT Aldo-keto reductase family 1 member B7 (Gene Name=Akr1b7)

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