Structure of PDB 3o1t Chain A

Receptor sequence
>3o1tA (length=202) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
EPLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMT
NCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDF
QPDACLINRYAPGAKLCLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRN
DPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQA
GK
3D structure
PDB3o1t Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase.
ChainA
Resolution1.48 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.14.11.33: DNA oxidative demethylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A T51 P52 G53 Y55 M57 W69 Y76 L118 K127 C129 H131 T39 P40 G41 Y43 M45 W57 Y64 L106 K115 C117 H119
BS02 dna A R161 N162 R167 R149 N150 R155
BS03 FE A H131 D133 H187 H119 D121 H175
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008198 ferrous iron binding
GO:0035515 oxidative RNA demethylase activity
GO:0035516 broad specificity oxidative DNA demethylase activity
GO:0046872 metal ion binding
GO:0051213 dioxygenase activity
Biological Process
GO:0006281 DNA repair
GO:0006307 DNA alkylation repair
GO:0035513 oxidative RNA demethylation
GO:0042245 RNA repair
GO:0070989 oxidative demethylation
GO:0072702 response to methyl methanesulfonate
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Biological Process
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Cellular Component
External links
PDB RCSB:3o1t, PDBe:3o1t, PDBj:3o1t
PDBsum3o1t
PubMed21068844
UniProtP05050|ALKB_ECOLI Alpha-ketoglutarate-dependent dioxygenase AlkB (Gene Name=alkB)

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