Structure of PDB 3nsj Chain A

Receptor sequence
>3nsjA (length=530) Species: 10090 (Mus musculus) [Search protein sequence]
PCYTATRSECKQKHKFVPGVWMAGEGMDVTTLRRSGSFPVNTQRFLRPDR
TCTLCKNSLMRDATQRLPVAITHWRPHSSHCQRNVAAAKVHSTEGVAREA
AANINNDWRVGLDVNRPEANMRASVAGSHSKVANFAAEKTYQDQYNFNSD
TVECRMYSFRLVQKPPLHLDFKKALRALPRNFNSSTEHAYHELISSYGTH
FITAVDLGGRISVLTALRTCQLTLNGLTADEVGDCLNVEAQVSIGAQASV
SSEYKACEEKKKQHKMATSFHQTYRERHVEVLGGPLDSTHDLLFGNQATP
EQFSTWTASLPSNPGLVDYSLEPLHTLLEEQNPKREALRQAISHYIMSRA
RWQNCSRPCRSGQHKSSHDSCQCECQDSKVTNQDCCPRQRGLAHLVVSNF
RAEHLWGDYTTATDAYLKVFFGGQEFRTGVVWNNNNPRWTDKMDFENVLL
STGGPLRVQVWDADYGWDDDLLGSCDRSPHSGFHEVTCELNHGRVKFSYH
AKCLPHLTGGTCLEYAPQGLLGDPPGNRSG
3D structure
PDB3nsj The structural basis for membrane binding and pore formation by lymphocyte perforin.
ChainA
Resolution2.75 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CA A D485 W488 D490 D464 W467 D469
BS02 CA A D435 D483 A484 D414 D462 A463
Gene Ontology
Molecular Function
GO:0005509 calcium ion binding
GO:0022829 wide pore channel activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding
GO:0140911 pore-forming activity
Biological Process
GO:0001771 immunological synapse formation
GO:0001909 leukocyte mediated cytotoxicity
GO:0001913 T cell mediated cytotoxicity
GO:0002357 defense response to tumor cell
GO:0002418 immune response to tumor cell
GO:0009306 protein secretion
GO:0017038 protein import
GO:0031640 killing of cells of another organism
GO:0051260 protein homooligomerization
GO:0051607 defense response to virus
GO:0051712 positive regulation of killing of cells of another organism
GO:0071806 protein transmembrane transport
GO:0140507 granzyme-mediated programmed cell death signaling pathway
Cellular Component
GO:0001772 immunological synapse
GO:0005576 extracellular region
GO:0005764 lysosome
GO:0005768 endosome
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0031410 cytoplasmic vesicle
GO:0031904 endosome lumen
GO:0044194 cytolytic granule

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3nsj, PDBe:3nsj, PDBj:3nsj
PDBsum3nsj
PubMed21037563
UniProtP10820|PERF_MOUSE Perforin-1 (Gene Name=Prf1)

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