Structure of PDB 3nit Chain A

Receptor sequence

>3nitA (length=89) Species: 4932 (Saccharomyces cerevisiae)

GSIDESRIGDVHKHTGRNCGRKFKIGEPLYRCHECGCDDTCVLCIHCFNP
KDHVNHHVCTDICTEFTSGICDCGDEEAWNSPLHCKAEE

3D structure

• PDB: 3nit Structural basis for the recognition of N-end rule substrates by the UBR box of ubiquitin ligases
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 2.3.2.27: RING-type E3 ubiquitin transferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C123 C148 C151 C175	C19 C44 C47 C71
BS02	ZN	A	H118 C151 C177 C189	H14 C47 C73 C85
BS03	ZN	A	C136 C139 H157 H160	C32 C35 H53 H56

Gene Ontology

Molecular Function

• GO:0008270 zinc ion binding
• GO:0061630 ubiquitin protein ligase activity

Biological Process

• GO:0071596 ubiquitin-dependent protein catabolic process via the N-end rule pathway

External links

• PDB: RCSB:3nit, PDBe:3nit, PDBj:3nit
• PDBsum: 3nit
• PubMed: 20835240
• UniProt: P19812|UBR1_YEAST E3 ubiquitin-protein ligase UBR1 (Gene Name=UBR1)