Structure of PDB 3mqk Chain A

Receptor sequence
>3mqkA (length=328) Species: 2261 (Pyrococcus furiosus) [Search protein sequence]
RILPADIKREVLIKDENAETNPDWGFPPEKRPIEMHIQFGVINLDKPPGP
TSHEVVAWIKKILNLEKAGHGGTLDPKVSGVLPVALEKATRVVQALLPAG
KEYVALMHLHGDVPEDKIIQVMKEFEGEIIQRPPLRSAVKRRLRTRKVYY
IEVLEIEGRDVLFRVGVEAGTYIRSLIHHIGLALGVGAHMSELRRTRSGP
FKEDETLITLHDLVDYYYFWKEDGIEEYFRKAIQPMEKAVEHLPKVWIKD
SAVAAVTHGADLAVPGIAKLHAGIKRGDLVAIMTLKDELVALGKAMMTSQ
EMLEKTKGIAVDVEKVFMPRDWYPKLWE
3D structure
PDB3mqk Structural and functional evidence of high specificity of Cbf5 for ACA trinucleotide.
ChainA
Resolution2.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D85 Y113 R184
Catalytic site (residue number reindexed from 1) D75 Y103 R174
Enzyme Commision number 5.4.99.25: tRNA pseudouridine(55) synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna A S261 A262 A265 H268 G269 A270 L272 A273 P275 G276 K317 G318 K335 W337 S251 A252 A255 H258 G259 A260 L262 A263 P265 G266 K307 G308 K325 W327
BS02 rna A K77 G269 V323 K325 V326 K67 G259 V313 K315 V316
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0009982 pseudouridine synthase activity
GO:0016853 isomerase activity
GO:0160148 tRNA pseudouridine(55) synthase activity
Biological Process
GO:0000495 box H/ACA sno(s)RNA 3'-end processing
GO:0001522 pseudouridine synthesis
GO:0006396 RNA processing
GO:0008033 tRNA processing
GO:0009451 RNA modification
GO:0031118 rRNA pseudouridine synthesis
GO:0031119 tRNA pseudouridine synthesis
GO:0031120 snRNA pseudouridine synthesis
GO:1990481 mRNA pseudouridine synthesis

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Molecular Function
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Biological Process
External links
PDB RCSB:3mqk, PDBe:3mqk, PDBj:3mqk
PDBsum3mqk
PubMed21149572
UniProtQ7LWY0|TRUB_PYRFU Probable tRNA pseudouridine synthase B (Gene Name=truB)

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