Structure of PDB 3mn5 Chain A

Receptor sequence

>3mn5A (length=347) Species: 9986 (Oryctolagus cuniculus) [Search protein sequence]

TTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRVGDERTLKYPIEHGIIT
NWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETF
NVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMR
LDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEM
ATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHET
TYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKI
KIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKC

3D structure

PDB

3mn5 Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation.

Chain

Resolution

1.5 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.6.4.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	G23 D24 D25 Y143 G146 T148 E167 S348 T351 Q354	G19 D20 D21 Y116 G119 T121 E140 S321 T324 Q327
BS02	ATP	A	G13 S14 G15 K18 G156 D157 V159 G182 R210 K213 E214 G302 M305 Y306 K336	G9 S10 G11 K14 G129 D130 V132 G155 R183 K186 E187 G275 M278 Y279 K309
BS03	LAB	A	G15 L16 P32 Y69 D157 R183 T186 R206 E207 R210	G11 L12 P28 Y42 D130 R156 T159 R179 E180 R183	BindingDB: IC50=8470nM

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003785	actin monomer binding
GO:0005509	calcium ion binding
GO:0005515	protein binding
GO:0005523	tropomyosin binding
GO:0005524	ATP binding
GO:0016787	hydrolase activity
GO:0019904	protein domain specific binding
GO:0031013	troponin I binding
GO:0031432	titin binding
GO:0032036	myosin heavy chain binding
GO:0042802	identical protein binding
GO:0048306	calcium-dependent protein binding
GO:0140660	cytoskeletal motor activator activity

	Biological Process
GO:0010628	positive regulation of gene expression
GO:0030041	actin filament polymerization
GO:0030240	skeletal muscle thin filament assembly
GO:0048741	skeletal muscle fiber development
GO:0051017	actin filament bundle assembly
GO:0090131	mesenchyme migration

	Cellular Component
GO:0001725	stress fiber
GO:0005737	cytoplasm
GO:0005856	cytoskeleton
GO:0005865	striated muscle thin filament
GO:0005884	actin filament
GO:0030027	lamellipodium
GO:0030175	filopodium
GO:0031941	filamentous actin
GO:0032432	actin filament bundle
GO:0044297	cell body
GO:0098723	skeletal muscle myofibril

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:3mn5, PDBe:3mn5, PDBj:3mn5
PDBsum	3mn5
PubMed	20538977
UniProt	P68135\|ACTS_RABIT Actin, alpha skeletal muscle (Gene Name=ACTA1)

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