Structure of PDB 3ml5 Chain A

Receptor sequence
>3ml5A (length=262) Species: 9606 (Homo sapiens) [Search protein sequence]
GHHGWGYGQDDGPSHWHKLYPIAQGDRQSPINIISSQAVYSPSLQPLELS
YEACMSLSITNNGHSVQVDFNDSDDRTVVTGGPLEGPYRLKQFHFHWGKK
HDVGSEHTVDGKSFPSELHLVHWNAKKYSTFGEAASAPDGLAVVGVFLET
GDEHPSMNRLTDALYMVRFKGTKAQFSCFNPKSLLPASRHYWTYPGSLTT
PPLSESVTWIVLREPISISERQMGKFRSLLFTSEDDERIHMVNNFRPPQP
LKGRVVKASFRA
3D structure
PDB3ml5 Crystal structure of the C183S/C217S mutant of human CA VII in complex with acetazolamide
ChainA
Resolution2.05 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H64 H94 H96 E106 H119 T199
Catalytic site (residue number reindexed from 1) H64 H94 H96 E106 H119 T199
Enzyme Commision number 4.2.1.1: carbonic anhydrase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A H94 H96 H119 H94 H96 H119
BS02 AZM A H94 V121 F131 L198 T199 T200 H94 V121 F131 L198 T199 T200 PDBbind-CN: -logKd/Ki=8.52,Ki=3.0nM
BindingDB: Ki=2.5nM,Kd=17nM,IC50=3440nM
Gene Ontology
Molecular Function
GO:0004089 carbonate dehydratase activity
GO:0008270 zinc ion binding
GO:0016829 lyase activity
GO:0046872 metal ion binding
Biological Process
GO:0006730 one-carbon metabolic process
GO:0032230 positive regulation of synaptic transmission, GABAergic
GO:0032849 positive regulation of cellular pH reduction
GO:0051453 regulation of intracellular pH
GO:0070050 neuron cellular homeostasis
GO:2001225 regulation of chloride transport
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:3ml5, PDBe:3ml5, PDBj:3ml5
PDBsum3ml5
PubMed20688517
UniProtP43166|CAH7_HUMAN Carbonic anhydrase 7 (Gene Name=CA7)

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