Structure of PDB 3mk2 Chain A

Receptor sequence
>3mk2A (length=481) Species: 9606 (Homo sapiens) [Search protein sequence]
IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVT
AARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYL
CGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR
VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVIL
GGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNR
TELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLS
RNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDT
LSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYV
LKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVH
GVQEQTFIAHVMAFAACLEPYTACDLAPPAG
3D structure
PDB3mk2 Refined structures of placental alkaline phosphatase show a consistent pattern of interactions at the peripheral site.
ChainA
Resolution1.89 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D42 S92 H153 S155 R166 E311 D316 H317 H320 D357 H358 H432
Catalytic site (residue number reindexed from 1) D42 S92 H153 S155 R166 E311 D316 H317 H320 D357 H358 H432
Enzyme Commision number 3.1.3.1: alkaline phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PHE A S92 F107 H320 H432 S92 F107 H320 H432
BS02 PHE A L253 M254 E290 E293 L253 M254 E290 E293
BS03 ZN A S92 D316 H320 H432 S92 D316 H320 H432
BS04 ZN A D42 S92 D357 H358 D42 S92 D357 H358
BS05 MG A D42 S155 E311 D42 S155 E311
BS06 CA A E216 F269 E270 D285 E216 F269 E270 D285
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004035 alkaline phosphatase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016791 phosphatase activity
GO:0046872 metal ion binding
Cellular Component
GO:0005886 plasma membrane
GO:0009986 cell surface
GO:0098552 side of membrane

View graph for
Molecular Function
View graph for
Cellular Component
External links
PDB RCSB:3mk2, PDBe:3mk2, PDBj:3mk2
PDBsum3mk2
PubMed20693656
UniProtP05187|PPB1_HUMAN Alkaline phosphatase, placental type (Gene Name=ALPP)

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