Structure of PDB 3mi3 Chain A

Receptor sequence
>3mi3A (length=370) Species: 4896 (Schizosaccharomyces pombe) [Search protein sequence]
NPSDFLSRVNNFSIIESTLREGEQFANAFFDTEKKIQIAKALDNFGVDYI
ELTSPVASEQSRQDCEAICKLGLKCKILTHIRCHMDDARVAVETGVDGVD
VVIGTSMTYIIDSATEVINFVKSKGIEVRFSSEDSFRSDLVDLLSLYKAV
DKIGVNRVGIADTVGCATPRQVYDLIRTLRGVVSCDIECHFHNDTGMAIA
NAYCALEAGATHIDTSILGIGERNGITPLGALLARMYVTDREYITHKYKL
NQLRELENLVADAVEVQIPFNNYITGMCAFTHKAGIHAKAILANPSTYEI
LKPEDFGMSRYVHVGSRLTGWNAIKSRAEQLNLHLTDAQAKELTVRIKKL
ADVRTLAMDDVDRVLREYHA
3D structure
PDB3mi3 Structural Basis for L-lysine Feedback Inhibition of Homocitrate Synthase
ChainA
Resolution2.38 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Q47
Catalytic site (residue number reindexed from 1) Q24
Enzyme Commision number 2.3.3.14: homocitrate synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 LYS A R43 D123 T197 H224 H226 R20 D100 T163 H190 H192 MOAD: Ki=3.56uM
PDBbind-CN: -logKd/Ki=5.45,Ki=3.56uM
BS02 ZN A E44 H224 H226 E21 H190 H192
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004410 homocitrate synthase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding
GO:0046912 acyltransferase activity, acyl groups converted into alkyl on transfer
Biological Process
GO:0009085 lysine biosynthetic process
GO:0019752 carboxylic acid metabolic process
GO:0019878 lysine biosynthetic process via aminoadipic acid
GO:0043436 oxoacid metabolic process
GO:0044283 small molecule biosynthetic process
Cellular Component
GO:0005634 nucleus
GO:0005739 mitochondrion
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3mi3, PDBe:3mi3, PDBj:3mi3
PDBsum3mi3
PubMed20089861
UniProtQ9Y823|HOSM_SCHPO Homocitrate synthase, mitochondrial (Gene Name=lys4)

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