Structure of PDB 3mgi Chain A

Receptor sequence

>3mgiA (length=319) Species: 9606 (Homo sapiens)

HNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACS
IPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQM
WYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQK
AAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSL
RQEGFLTDDLVKGETKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYF
TGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPTE
KDVFRLLGLPYREPAERDW

3D structure

• PDB: 3mgi Loop 1 modulates the fidelity of DNA polymerase lambda
• Chain: A
• Resolution: 2.603 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D176 D178 D234
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 K463 G464 T471 K472 R514 R517 K521 L527 S528 E529 H530 H541 K544	W23 V211 K212 G213 T215 K216 R258 R261 K265 L271 S272 E273 H274 H285 K288
BS02	dna	A	W342 G343 G345 K347 T348 D490 Y505	W91 G92 G94 K96 T97 D234 Y249
BS03	dna	A	Y267 W274 R275 G278 Y279 P303 G304 G306 R308 M309 K312	Y16 W23 R24 G27 Y28 P52 G53 G55 R57 M58 K61
BS04	D3T	A	R386 G416 S417 R420 C425 G426 D427 D429 Y505 G508 A510 N513	R135 G165 S166 R169 C174 G175 D176 D178 Y249 G252 A254 N257
BS05	MG	A	D427 D429	D176 D178

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3mgi, PDBe:3mgi, PDBj:3mgi
• PDBsum: 3mgi
• PubMed: 20435673
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)