Structure of PDB 3mfi Chain A

Receptor sequence

>3mfiA (length=511) Species: 4932 (Saccharomyces cerevisiae)

DPHMSKFTWKELIQLGSPSKAYESSLACIAHIDMNAFFAQVEQMRCGLSK
EDPVVCVQWNSIIAVSYAARKYGISRMDTIQEALKKCSNLIPIHTAVFKK
GEDFWQYHDGCGSWVQDPAKQISVEDHKVSLEPYRRESRKALAIFKWACD
LVERASIDEVFLDLGRICFNMLMFDNEYELTGDLKLKDALSNIREAFIGG
NYDINSHLPLIPEKIKSLKFEGDVFNPEGRDLITDWDDVILALGSQVCKG
IRDSIKDILGYTTSCGLSSTKNVCKLASNYKKPDAQTIVKNDCLLDFLDC
GKFEITSFWTLGGVLGKELIDVLDLPHENSIKHIRETWPDNAGQLKEFLD
AKVKQSDYDSNIDPLKTADLAEKLFKLSRGRYGLPLSSRPVVKSMMSNKN
LRGKSCNSIVDCISWLEVFCAELTSRIQDLEQEYNKIVIPRTVSISLKTK
SYEVYRKSGPVAYKGINFQSHELLKVGIKFVTDLDIKGKNKSYYPLTKLS
MTITNFDIIDL

3D structure

• PDB: 3mfi Structural basis for the suppression of skin cancers by DNA polymerase eta.
• Chain: A
• Resolution: 1.76 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	K272 W306 T307 G309 G310 V311 L312 R456 K457 S458	K275 W309 T310 G312 G313 V314 L315 R456 K457 S458
BS02	dna	A	Q55 W56 M74 K125 R132 R389 K393 S394 M395 M396 N398 N400 R426 Y452	Q58 W59 M77 K128 R135 R389 K393 S394 M395 M396 N398 N400 R426 Y452
BS03	DTP	A	D30 M31 N32 A33 F34 F35 Y64 R73 D155 K279	D33 M34 N35 A36 F37 F38 Y67 R76 D158 K282
BS04	MG	A	D30 M31 D155	D33 M34 D158

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3mfi, PDBe:3mfi, PDBj:3mfi
• PDBsum: 3mfi
• PubMed: 20577207
• UniProt: Q04049|POLH_YEAST DNA polymerase eta (Gene Name=RAD30)