Structure of PDB 3mfh Chain A

Receptor sequence

>3mfhA (length=510) Species: 4932 (Saccharomyces cerevisiae)

MSKFTWKELIQLGSPSKAYESSLACIAHIDMNAFFAQVEQMRCGLSKEDP
VVCVQWNSIIAVSYAARKYGISRMDTIQEALKKCSNLIPIHTAVFKKGED
FWQYHDGCGSWVPAKQISVEDHKVSLEPYRRESRKALAIFKWACDLVERA
SIDEVFLDLGRICFNMLMFDNEYELTGDLKLKDALSNIREAFIGGNYDIN
SHLPLIPEKIKSLKFEGDVFNPEGRDLITDWDDVILALGSQVCKGIRDSI
KDILGYTTSCGLSSTKNVCKLASNYKKPDAQTIVKNDCLLDFLDCGKFEI
TSFWTLGGVLGKELIDVLDLPHENSIKHIRETWPDNAGQLKEFLDAKVKQ
SDYDRSTSNIDPLKTADLAEKLFKLSRGRYGLPLSSRPVVKSMMSNKNLR
GKSCNSIVDCISWLEVFCAELTSRIQDLEQEYNKIVIPRTVSISLKTKSY
EVYRKSGPVAYKGINFQSHELLKVGIKFVTDLDIKGKNKSYYPLTKLSMT
ITNFDIIDLQ

3D structure

• PDB: 3mfh Structural basis for the suppression of skin cancers by DNA polymerase eta.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E156 K272 W306 T307 G309 G310 V311 L312 R456 K457 S458 G459	E154 K270 W304 T305 G307 G308 V309 L310 R454 K455 S456 G457
BS02	dna	A	Q55 W56 R73 M74 K125 V126 R132 R389 K393 S394 M395 M396 N398 N400 R426	Q55 W56 R73 M74 K123 V124 R130 R387 K391 S392 M393 M394 N396 N398 R424
BS03	DTP	A	D30 M31 N32 A33 F34 F35 I60 Y64 R67 R73 D155 K279	D30 M31 N32 A33 F34 F35 I60 Y64 R67 R73 D153 K277
BS04	MG	A	D30 M31 D155	D30 M31 D153

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3mfh, PDBe:3mfh, PDBj:3mfh
• PDBsum: 3mfh
• PubMed: 20577207
• UniProt: Q04049|POLH_YEAST DNA polymerase eta (Gene Name=RAD30)