Structure of PDB 3mb5 Chain A

Receptor sequence
>3mb5A (length=255) Species: 29292 (Pyrococcus abyssi) [Search protein sequence]
MIREGDKVVLVDPRGKRYLITVSKRDFHTDLGILKLEEIIGRNFGEAIKS
HKGHEFKILRPRIVDYLDKMKRGPQIVHPKDAALIVAYAGISPGDFIVEA
GVGSGALTLFLANIVGPEGRVVSYEIREDFAKLAWENIKWAGFDDRVTIK
LKDIYEGIEEENVDHVILDLPQPERVVEHAAKALKPGGFFVAYTPCSNQV
MRLHEKLREFKDYFMKPRTINVLVFDQEVKKECMRPRTTALVHTGYITFA
RRILE
3D structure
PDB3mb5 Insights into the hyperthermostability and unusual region-specificity of archaeal Pyrococcus abyssi tRNA m1A57/58 methyltransferase.
ChainA
Resolution1.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.219: tRNA (adenine(57)-N(1)/adenine(58)-N(1))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAM A Q75 I76 V77 G101 G103 S104 A106 L107 E125 I126 D153 I154 Y155 D169 Q75 I76 V77 G101 G103 S104 A106 L107 E125 I126 D153 I154 Y155 D169
Gene Ontology
Molecular Function
GO:0008168 methyltransferase activity
GO:0016740 transferase activity
GO:0043827 tRNA (adenine(57)-N1)/(adenine(58)-N1)-methyltransferase activity
GO:0160107 tRNA (adenine(58)-N1)-methyltransferase activity
Biological Process
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0032259 methylation
Cellular Component
GO:0031515 tRNA (m1A) methyltransferase complex

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Biological Process
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Cellular Component
External links
PDB RCSB:3mb5, PDBe:3mb5, PDBj:3mb5
PDBsum3mb5
PubMed20483913
UniProtQ9V1J7|TRMI_PYRAB tRNA (adenine(57)-N(1)/adenine(58)-N(1))-methyltransferase TrmI (Gene Name=trmI)

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