Structure of PDB 3m09 Chain A

Receptor sequence
>3m09A (length=161) Species: 1280 (Staphylococcus aureus) [Search protein sequence]
TLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESI
GKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLYEE
MIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHT
FLHLIRKAVPR
3D structure
PDB3m09 Inhibition of Antibiotic-Resistant Staphylococcus aureus by the Broad-Spectrum Dihydrofolate Reductase Inhibitor RAB1.
ChainA
Resolution2.009 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) L5 L20 W22 D27 L28 V31 L54 V89 T111
Catalytic site (residue number reindexed from 1) L5 L20 W22 D27 L28 V31 L54 V89 T111
Enzyme Commision number 1.5.1.3: dihydrofolate reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 RAR A L5 V6 A7 D27 L28 K29 V31 K32 P55 R57 F92 L5 V6 A7 D27 L28 K29 V31 K32 P55 R57 F92 MOAD: Ki=0.9nM
BindingDB: IC50=12.5nM,Ki=0.9nM
BS02 NAP A V6 A7 I14 G15 N18 Q19 L20 G43 R44 K45 T46 L62 T63 S64 F92 G94 Q95 T96 E100 T121 V6 A7 I14 G15 N18 Q19 L20 G43 R44 K45 T46 L62 T63 S64 F92 G94 Q95 T96 E100 T121
Gene Ontology
Molecular Function
GO:0004146 dihydrofolate reductase activity
GO:0016491 oxidoreductase activity
GO:0050661 NADP binding
Biological Process
GO:0006730 one-carbon metabolic process
GO:0046452 dihydrofolate metabolic process
GO:0046654 tetrahydrofolate biosynthetic process
GO:0046655 folic acid metabolic process
Cellular Component
GO:0005829 cytosol

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Biological Process
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Cellular Component
External links
PDB RCSB:3m09, PDBe:3m09, PDBj:3m09
PDBsum3m09
PubMed20606069
UniProtP0A017|DYR_STAAU Dihydrofolate reductase (Gene Name=folA)

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