Structure of PDB 3lya Chain A

Receptor sequence

>3lyaA (length=318) Species: 511145 (Escherichia coli str. K-12 substr. MG1655)

ILLNPRDIDINMVNKSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHD
KINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSIKLVDNADGSTMLDKR
YVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQKILPHRGALLTNFY
QAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHP
LDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAIN
TGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIEN
GIFQTSVPYVVPYLDKFL

3D structure

• PDB: 3lya Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q342 L355 E461 N466 R467
• Catalytic site (residue number reindexed from 1): Q151 L164 E270 N275 R276

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	RHE	A	H332 R333 L336 E371 F372	H141 R142 L145 E180 F181
BS02	RHE	A	N356 R357 E360	N165 R166 E169
BS03	RHE	A	D225 T229 H344 E447 S449 W450	D34 T38 H153 E256 S258 W259
BS04	RHE	A	N356 R386 H390 Q396	N165 R195 H199 Q205
BS05	RHE	A	Q396 A399	Q205 A208
BS06	RHE	A	V366 K379	V175 K188
BS07	RHE	A	N246 I247 F477 G482 A483 L509	N55 I56 F286 G291 A292 L318
BS08	RHE	A	D352 K353	D161 K162
BS09	RHE	A	A470 V502 P503 Y504	A279 V311 P312 Y313
BS10	RHE	A	E490 Q495	E299 Q304
BS11	RHE	A	N244 D345 H349	N53 D154 H158
BS12	RHE	A	L307 E308 W318 Q323	L116 E117 W127 Q132
BS13	RHE	A	K423 S426 A427 K430	K232 S235 A236 K239
BS14	RHE	A	E324 N415	E133 N224
BS15	RHE	A	D393 E394 K395	D202 E203 K204

External links

• PDB: RCSB:3lya, PDBe:3lya, PDBj:3lya
• PDBsum: 3lya
• PubMed: 21308846
• UniProt: P23890|CADC_ECOLI Transcriptional activator CadC (Gene Name=cadC)