Structure of PDB 3lwl Chain A

Receptor sequence

>3lwlA (length=534) Species: 271 (Thermus aquaticus) [Search protein sequence]

EEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRD
LKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRY
GGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHM
EATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVL
FDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKST
YIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRI
RRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETAS
WMFGVPRELMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQ
SFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERMA
FNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEA
VARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

3D structure

PDB

3lwl Replication through an abasic DNA lesion: structural basis for adenine selectivity

Chain

Resolution

2.25 Å

3D
structure

[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]

Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R487 T506 K508 T509 S513 T514 S515 A516 R536 K540 Y545 R573 N583 I584 P585 R587 V783 H784 D785	R193 T212 K214 T215 S219 T220 S221 A222 R242 K246 Y251 R279 N289 I290 P291 R293 V485 H486 D487	PDBbind-CN: Kd=149uM
BS02	dna	A	N483 N485 S486 S543 T544 T569 A570 T571 S575 S576 S577 D578 N580 Y671 M673 S674 H676 R728 R746 M747 N750 Q754	N189 N191 S192 S249 T250 T275 A276 T277 S281 S282 S283 D284 N286 Y373 M375 S376 H378 R430 R448 M449 N452 Q456	PDBbind-CN: Kd=149uM
BS03	DDS	A	R587 Q613 H639 R659 K663 F667 Y671	R293 Q319 H345 R361 K365 F369 Y373

Gene Ontology

	Molecular Function
GO:0001882	nucleoside binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006281	DNA repair

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:3lwl, PDBe:3lwl, PDBj:3lwl
PDBsum	3lwl
PubMed	20400942
UniProt	P19821\|DPO1_THEAQ DNA polymerase I, thermostable (Gene Name=polA)

[Back to BioLiP]