Structure of PDB 3lsm Chain A

Receptor sequence

>3lsmA (length=574) Species: 230624 (Trametes ochracea)

IKYDVVIVGSGPIGCTYARELVGAGYKVAMFDIGEIDSGLKIGAHKKNTV
EYQKNIDKFVNVIQGQLMSVSVPVNTLVVDTLSPTSWQASTFFVRNGSNP
EQDPLRNLSGQAVTRVVGGMSTAWTCATPRFDREQRPLLVKDDADADDAE
WDRLYTKAESYFQTGTDQFKESIRHNLVLNKLTEEYKGQRDFQQIPLAAT
RRSPTFVEWSSANTVFDLQNRPNTDAPEERFNLFPAVACERVVRNALNSE
IESLHIHDLISGDRFEIKADVYVLTAGAVHNTQLLVNSGFGQLGRPNPAN
PPELLPSLGSYITEQSLVFCQTVMSTELIDSVKSDMTIRGTPGELTYSVT
YTPGASTNKHPDWWNEKVKNHMMQHQEDPLPIPFEDPEPQVTTLFQPSHP
WHTQIHRDAFSYGAVQQSIDSRLIVDWRFFGRTEPKEENKLWFSDKITDA
YNMPQPTFDFRFPAGRTSKEAEDMMTDMCVMSAKIGGFLPGSLPQFMEPG
LVLHLGGTHRMGFDEKEDNCCVNTDSRVFGFKNLFLGGCGNIPTAYGANP
TLTAMSLAIKSCEYIKQNFTPSPF

3D structure

• PDB: 3lsm H-bonding and positive charge at the N5/O4 locus are critical for covalent flavin attachment in trametes pyranose 2-oxidase.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 1.1.3.10: pyranose oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SFD	A	G55 P56 I57 D76 I77 T158 G163 M164 W168 T169 C170 A171 C283 A320 H324 R472 H548 N593 T595	G11 P12 I13 D32 I33 T114 G119 M120 W124 T125 C126 A127 C239 A276 H280 R428 H504 N549 T551
BS02	SO3	A	R571 G574	R527 G530
BS03	SO3	A	S293 K576	S249 K532
BS04	GLC	A	R63 E64 K201 Y205 K610	R19 E20 K157 Y161 K566

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity
• GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
• GO:0050233 pyranose oxidase activity
• GO:0050660 flavin adenine dinucleotide binding

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:3lsm, PDBe:3lsm, PDBj:3lsm
• PDBsum: 3lsm
• PubMed: 20708626
• UniProt: Q7ZA32