Structure of PDB 3kql Chain A

Receptor sequence

>3kqlA (length=437) Species: 333284 (Hepatitis C virus (isolate Con1))

SPPAVPQTFQVAHLHAPTGSGKSTKVPAAYAAQGYKVLVLNPSVAATLGF
GAYMSKAHGIDPNIRTGVRTITTGAPITYSTYGKFLADGGCSGGAYDIII
CDECHSTDSTTILGIGTVLDQAETAGARLVVLATATPPGSVTVPHPNIEE
VALSSTGEIPFYGKAIPIETIKGGRHLIFCHSKKKCDELAAKLSGLGLNA
VAYYRGLDVSVIPTSGDVIVVATDALMTGFTGDFDSVIDCNTCVTQTVDF
SLDPTFTIETTTVPQDAVSRSQRRGRTGRGRMGIYRFVTPGERPSGMFDS
SVLCECYDAGCAWYELTPAETSVRLRAYLNTPGLPVCQDHLEFWESVFTG
LTHIDAHFLSQTKQAGDNFPYLVAYQATVCARAQAPPPSWDQMWKCLIRL
KPTLHGPTPLLYRLGAVQNEVTTTHPITKYIMACMSA

3D structure

• PDB: 3kql Three conformational snapshots of the hepatitis C virus NS3 helicase reveal a ratchet translocation mechanism.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.98: hepacivirin.
  3.4.22.-
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	P230 V232 T254 G255 T269 K272 H369 K371 Y392 R393 T411 A413 V432 Q434 T448 W501 Y502 K551	P42 V44 T66 G67 T81 K84 H181 K183 Y204 R205 T223 A225 V244 Q246 T260 W313 Y314 K363
BS02	ALF	A	T206 G207 K210 E291 G417 R464 R467	T18 G19 K22 E103 G229 R276 R279
BS03	ADP	A	G207 S208 G209 K210 S211 T212 Y241 T419 R467 G468	G19 S20 G21 K22 S23 T24 Y53 T231 R279 G280

Gene Ontology

Molecular Function

• GO:0004386 helicase activity
• GO:0005524 ATP binding

External links

• PDB: RCSB:3kql, PDBe:3kql, PDBj:3kql
• PDBsum: 3kql
• PubMed: 20080715
• UniProt: Q9WMX2|POLG_HCVCO Genome polyprotein