Structure of PDB 3kjm Chain A

Receptor sequence

>3kjmA (length=496) Species: 4577 (Zea mays)

PWPASLAALALDGKLRTDSNATAAASTDFGNITSALPAAVLYPSSTADLV
ALLSAANSTPGWPYTIAFRGRGHSLMGQAFAPGGVVVNMASLGDAPPRIN
VSADGRYVDAGGEQVWIDVLRASLARGVAPRSWTDYLYLTVGGTLSNAGI
SGQAFRHGPQISNVLEMDVITGHGEMVTCSKQLNADLFDAVLGGLGQFGV
ITRARIAVEPAPARARWVRLVYTDFAAFSADQERLTAPRPASFGPMSYVE
GSVFVNQSLATDLANTGFFTDADVARIVALAGERNATTVYSIEATLNYDN
ATAAAAAVDQELASVLGTLSYVEGFAFQRDVAYAAFLDRVHGEEVALNKL
GLWRVPHPWLNMFVPRSRIADFDRGVFKGILQGTDIVGPLIVYPLNKSMW
DDGMSAATPSEDVFYAVSLLFSSVAPNDLARLQEQNRRILRFCDLAGIQY
KTYAARHTDRSDWVRHFGAAKWNRFVEMKNKYDPKRLLSPGQDIFN

3D structure

• PDB: 3kjm Phenyl- and benzylurea cytokinins as competitive inhibitors of cytokinin oxidase/dehydrogenase: a structural study.
• Chain: A
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H105 D169 E288
• Catalytic site (residue number reindexed from 1): H73 D135 E250
• Enzyme Commision number: 1.5.99.12: cytokinin dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	F61 F100 G102 R103 G104 H105 S106 Q110 T168 D169 Y170 L173 T174 G176 G177 S180 N181 G183 I184 V234 I235 W397 Y491 S527	F29 F68 G70 R71 G72 H73 S74 Q78 T134 D135 Y136 L139 T140 G142 G143 S146 N147 G149 I150 V200 I201 W359 Y453 S489
BS02	245	A	D169 V378 W397 N399 I429 L458 Y491	D135 V340 W359 N361 I391 L420 Y453	MOAD: ic50=1.5uM

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0019139 cytokinin dehydrogenase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0009690 cytokinin metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3kjm, PDBe:3kjm, PDBj:3kjm
• PDBsum: 3kjm
• PubMed: 20478354
• UniProt: Q9T0N8|CKX1_MAIZE Cytokinin dehydrogenase 1 (Gene Name=CKX1)