Structure of PDB 3khu Chain A

Receptor sequence

>3khuA (length=463) Species: 9606 (Homo sapiens) [Search protein sequence]

SMFEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPT
LPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMG
KGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTK
PNLNLQVLSNPQFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVY
EHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVE
EVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARY
WQQVIDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESS
SIYISKYLMDEGAHLHIYDPKVPREQIVVDLSHPDDQVSRLVTISKDPYE
ACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQ
TIGFQIETIGKKV

3D structure

PDB

3khu Structural and Kinetic Evidence That Catalytic Reaction of Human UDP-glucose 6-Dehydrogenase Involves Covalent Thiohemiacetal and Thioester Enzyme Intermediates.

Chain

Resolution

2.3 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	T131 E165 K220 N224 C276 D280
Catalytic site (residue number reindexed from 1)	T132 E166 K221 N225 C277 D281
Enzyme Commision number	1.1.1.22: UDP-glucose 6-dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UPG	A	F162 L163 A164 E165 K220 I231 F265 K267 S269 F272 G273 C276 F277 F338 K339	F163 L164 A165 E166 K221 I232 F266 K268 S270 F273 G274 C277 F278 F339 K340
BS02	UPG	A	G11 G13 Y14 V15 D36 V37 R41 V89 S130 T131 Q161 S275 R346	G12 G14 Y15 V16 D37 V38 R42 V90 S131 T132 Q162 S276 R347

Gene Ontology

	Molecular Function
GO:0003979	UDP-glucose 6-dehydrogenase activity
GO:0016491	oxidoreductase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0042802	identical protein binding
GO:0051287	NAD binding

	Biological Process
GO:0001702	gastrulation with mouth forming second
GO:0006024	glycosaminoglycan biosynthetic process
GO:0006065	UDP-glucuronate biosynthetic process
GO:0015012	heparan sulfate proteoglycan biosynthetic process
GO:0030206	chondroitin sulfate biosynthetic process
GO:0034214	protein hexamerization
GO:0048666	neuron development

	Cellular Component
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005829	cytosol
GO:0070062	extracellular exosome

View graph for
Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:3khu, PDBe:3khu, PDBj:3khu
PDBsum	3khu
PubMed	22123821
UniProt	O60701\|UGDH_HUMAN UDP-glucose 6-dehydrogenase (Gene Name=UGDH)

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