Structure of PDB 3k9y Chain A

Receptor sequence

>3k9yA (length=461) Species: 10116 (Rattus norvegicus) [Search protein sequence]

NVTDLPGPTNWPLLGSLLEIFWKGGLKKQHDTLAEYHKKYGQIFRMKLGS
FDSVHLGSPSLLEALYRTESAHPQRLEIKPWKAYRDHRNEAYGLMILEGQ
EWQRVRSAFQKKLMKPVEIMKLDKKINEVLADFLERMDELCDERGRIPDL
YSELNKWSFESICLVLYEKRFGLLQKETEEEALTFITAIKTMMSTFGKMM
VTPVELHKRLNTKVWQAHTLAWDTIFKSVKPCIDNRLQRYSQQPGADFLC
DIYQQDHLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQAQRRLL
QEVQSVLPDNQTPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKPTV
LGEYALPKGTVLTLNTQVLGSSEDNFEDSHKFRPERWLQKEKKINPFAHL
PFGIGKRMCIGRRLAELQLHLALCWIIQKYDIVATDNEPVEMLHLGILVP
SRELPIAFRPR

3D structure

PDB

3k9y Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism.

Chain

Resolution

2.8 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	T330 F455 C462
Catalytic site (residue number reindexed from 1)	T277 F402 C409
Enzyme Commision number	1.14.15.16: vitamin D3 24-hydroxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	HEM	A	R128 L147 M148 W155 R159 L323 A326 A327 T330 S334 T394 R396 P454 F455 G456 R460 M461 C462 I463 G464	R75 L94 M95 W102 R106 L270 A273 A274 T277 S281 T341 R343 P401 F402 G403 R407 M408 C409 I410 G411
BS02	CM5	A	M246 S247 F249	M193 S194 F196
BS03	CM5	A	F74 L79 Q82 F393	F21 L26 Q29 F340

Gene Ontology

	Molecular Function
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0008403	25-hydroxycholecalciferol-24-hydroxylase activity
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037	heme binding
GO:0030342	1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity
GO:0046872	metal ion binding
GO:0062180	25-hydroxycholecalciferol-23-hydroxylase activity
GO:0062181	1-alpha,25-dihydroxyvitamin D3 23-hydroxylase activity
GO:0070576	vitamin D 24-hydroxylase activity
GO:0070643	vitamin D 25-hydroxylase activity

	Biological Process
GO:0001649	osteoblast differentiation
GO:0006629	lipid metabolic process
GO:0008202	steroid metabolic process
GO:0033280	response to vitamin D
GO:0042359	vitamin D metabolic process
GO:0042369	vitamin D catabolic process
GO:0071305	cellular response to vitamin D

	Cellular Component
GO:0005739	mitochondrion

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:3k9y, PDBe:3k9y, PDBj:3k9y
PDBsum	3k9y
PubMed	19961857
UniProt	Q09128\|CP24A_RAT 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (Gene Name=Cyp24a1)

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