Structure of PDB 3ive Chain A

Receptor sequence

>3iveA (length=495) Species: 217992 (Escherichia coli O6)

AKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKA
TWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDNT
LLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIGVIGLHGVFA
FNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSS
MDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGG
IDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDQQTKQVIDGWNKKLD
EVVQQTVAQSPVELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGI
RNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASLSNGVLQ
VSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGF
TAFTEGKARNITGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDIK

3D structure

• PDB: 3ive Putative 5'-Nucleotidase (c4898) from Escherichia Coli in complex with Cytidine
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D32 H34 D79 N111 H112 D115 H212 H251 H253 Y342 R375
• Catalytic site (residue number reindexed from 1): D11 H13 D58 N90 H91 D94 H191 H227 H229 Y318 R351

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	A	D32 H34 D79 H253	D11 H13 D58 H229
BS02	FE	A	D79 N111 H212 H251	D58 N90 H191 H227
BS03	CTN	A	H112 T175 N370 G372 R375 F394 N396 F466 D472	H91 T154 N346 G348 R351 F370 N372 F442 D448

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0009166 nucleotide catabolic process

External links

• PDB: RCSB:3ive, PDBe:3ive, PDBj:3ive
• PDBsum: 3ive
• UniProt: A0A0H2VD83