Structure of PDB 3iv9 Chain A

Receptor sequence

>3iv9A (length=577) Species: 562 (Escherichia coli)

QAEWRSWEVNKRLEYSLVKGITEFIEQDTEEARQQATRPCEVIEGPLMDG
MNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASKEQCKTNGKMV
IATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNADL
IGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNY
SGPTVYVQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPR
TPPVTLEAARDNDFAFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPF
FMTWSLAGKYPRILEDEVVGVEAQRLFKDANDMLDKLSAEKTLNPRGVVG
LFPANRVGDDIEIYRDETRTHVINVSHHLRQQTEKTGFANYCLADFVAPK
LSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMVKALADRLAEAFA
EYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHTEKAT
IWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQV
EDYARRKGMSVTEVERWLAPNLGYDAD

3D structure

• PDB: 3iv9 Insights into the reactivation of cobalamin-dependent methionine synthase.
• Chain: A
• Resolution: 3.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D757 H759 S810
• Catalytic site (residue number reindexed from 1): D107 H109 S160
• Enzyme Commision number: 2.1.1.13: methionine synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	B12	A	V758 H759 I761 G762 I765 G802 S804 L806 T808 G833 G834 A835 V857 Q858 N859 A860 D1093 P1137 G1138 Y1139 H1145 M1171 G1174 A1175 S1176 V1177 S1178	V108 H109 I111 G112 I115 G152 S154 L156 T158 G183 G184 A185 V207 Q208 N209 A210 D443 P487 G488 Y489 H495 M521 G524 A525 S526 V527 S528

Gene Ontology

Molecular Function

• GO:0008705 methionine synthase activity
• GO:0031419 cobalamin binding
• GO:0046872 metal ion binding

Biological Process

• GO:0009086 methionine biosynthetic process

External links

• PDB: RCSB:3iv9, PDBe:3iv9, PDBj:3iv9
• PDBsum: 3iv9
• PubMed: 19846791
• UniProt: P13009|METH_ECOLI Methionine synthase (Gene Name=metH)