Structure of PDB 3igo Chain A

Receptor sequence

>3igoA (length=446) Species: 353152 (Cryptosporidium parvum Iowa II)

QGTFAERYNIVCMLGKGSFGEVLKCKDRITQQEYAVKVINKASAKNKTST
ILREVELLKKLDHPNIMKLFEILEDSSSFYIVGELYTGGELFDEIIKRKR
FSEHDAARIIKQVFSGITYMHKHNIVHRDLKPENILLESKEKDCDIKIID
FGLSTCFQQNTTAYYIAPEVLRGTYDEKCDVWSAGVILYILLSGTPPFYG
KNEYDILKRVETGKYAFDLPQWRTISDDAKDLIRKMLTFHPSLRITATQC
LEHPWIQKYSSETPTISDLPSLESAMTNIRQFQAEKKLAQAALLYMASKL
TTLDETKQLTEIFRKLDTNNDGMLDRDELVRGYHEFMRLKGVDSNSLIQG
STIEDQIDSLMPLLDMDGSGSIEYSEFIASAIDRTILLSRERMERAFKMF
DKDGSGKISTKELFKLESIIEQVDNNKDGEVDFNEFVEMLQNFVRN

3D structure

• PDB: 3igo Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.
• Chain: A
• Resolution: 2.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D198 K200 E202 N203 D219 T238
• Catalytic site (residue number reindexed from 1): D129 K131 E133 N134 D150 T162

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D515 N517 D519 E521 E526	D424 N426 D428 E430 E435
BS02	CA	A	D393 N395 D397 M399 E404	D317 N319 D321 M323 E328
BS03	CA	A	D443 D445 S447 S449 E451 E454	D365 D367 S369 S371 E373 E376
BS04	ANP	A	G83 G85 S86 F87 V90 K105 E153 Y155 L205 D219	G15 G17 S18 F19 V22 K37 E84 Y86 L136 D150
BS05	CA	A	D481 K489	D403 K411

Gene Ontology

Molecular Function

• GO:0004672 protein kinase activity
• GO:0005509 calcium ion binding
• GO:0005524 ATP binding

Biological Process

• GO:0006468 protein phosphorylation

External links

• PDB: RCSB:3igo, PDBe:3igo, PDBj:3igo
• PDBsum: 3igo
• PubMed: 20436473
• UniProt: A3FQ16