Structure of PDB 3ig8 Chain A

Receptor sequence

>3ig8A (length=672) Species: 4932 (Saccharomyces cerevisiae)

GLLALGTPLQWFESRTYNEHIRDEGIEQLLYIFQAAGKRDNDPLFWGDEL
EYMVVDFDDKERNSMLDVCHDKILTELNMEDSSLCEANDVSFHPEYGRYM
LEATPASPYLNYVGSYVEVNMQKRRAIAEYKLSEYARQDSKNNLHVGSRS
VPLTLTVFPRMGCPDFINIKDPWNHKNAASRSLFLPDEVINRHVRFPNLT
ASIRTRRGEKVCMNVPMYKDIATPETDDSIYDRDWFLPEDKEAKLASKPG
FIYMDSMGFGMGCSCLQVTFQAPNINKARYLYDALVNFAPIMLAFSAAAP
AFKGWLADQDVRWNVISGAVDDRTPKERGVAPLLPKYNKNGFGGIAKDVQ
DKVLEIPKSRYSSVDLFLGGSKFFNRTYNDTNVPINEKVLGRLLENDKAP
LDYDLAKHFAHLYIRDPVSTFEELLNQDNKTSSNHFENIQSTNWQTLRFK
PPTQQATPDKKDSPGWRVEFRPFEVQLLDFENAAYSVLIYLIVDSILTFS
DNINAYIHMSKVWENMKIAHHRDAILFEKFHWKKSFRNDTDVETEDYSIS
EIFHNPENGIFPQFVTPILCQKGFVTKDWKELKHSSKHERLYYYLKLISD
RASGELPTTAKFFRNFVLQHPDYKHDSKISKSINYDLLSTCDRLTHLDDS
KGELTSFLGAEIAEYVKKNKPS

3D structure

• PDB: 3ig8 Mechanistic details of glutathione biosynthesis revealed by crystal structures of Saccharomyces cerevisiae glutamate cysteine ligase.
• Chain: A
• Resolution: 2.69 Å

Enzymatic activity

• Enzyme Commision number: 6.3.2.2: glutamate--cysteine ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLU	A	E52 E96 C266 Q268 R313 I317	E51 E95 C265 Q267 R312 I316
BS02	ADP	A	F46 G48 D49 E50 T270 Q272 R468	F45 G47 D48 E49 T269 Q271 R467
BS03	MG	A	E52 E96 E103	E51 E95 E102
BS04	MG	A	E50 Q268 E470	E49 Q267 E469
BS05	MG	A	E50 E103	E49 E102

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004357 glutamate-cysteine ligase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity

Biological Process

• GO:0006750 glutathione biosynthetic process
• GO:0042542 response to hydrogen peroxide
• GO:0046686 response to cadmium ion

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3ig8, PDBe:3ig8, PDBj:3ig8
• PDBsum: 3ig8
• PubMed: 19726687
• UniProt: P32477|GSH1_YEAST Glutamate--cysteine ligase (Gene Name=GSH1)