Structure of PDB 3iem Chain A

Receptor sequence

>3iemA (length=431) Species: 300852 (Thermus thermophilus HB8)

MRIVPFGAAREVTGSAHLLLAGGRRVLLDCGMFQGKEEARNHAPFGFDPK
EVDAVLLTHAHLDHVGRLPKLFREGYRGPVYATRATVLLMEIVLEDALKV
MDEPFFGPEDVEEALGHLRPLEYGEWLRLGALSLAFGQAGHLPGSAFVVA
QGEGRTLVYSGDLGNREKDVLPDPSLPPLADLVLAEGTYGDRPHRPYRET
VREFLEILEKTLSQGGKVLIPTFAVERAQEILYVLYTHGHRLPRAPIYLD
SPMAGRVLSLYPRLVRYFSEEVQAHFLQGKNPFRPAGLEVVEHTEASKAL
NRAPGPMVVLAGSGMLAGGRILHHLKHGLSDPRNALVFVGYQPQGGLGAE
IIARPPAVRILGEEVPLRASVHTLGGFSGHAGQDELLDWLQGEPRVVLVH
GEEEKLLALGKLLALRGQEVSLARFGEGVPV

3D structure

• PDB: 3iem Crystal Structure of TTHA0252 from Thermus thermophilus HB8 complexed with RNA analog
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	T13 Q34 H61 L62 V100 T188 Y189 F223 A224 R227 P252 T294 S313 G314 M315 A317 R320 G340 Y341 S378 G379 H380 H400	T13 Q34 H61 L62 V100 T188 Y189 F223 A224 R227 P252 T294 S313 G314 M315 A317 R320 G340 Y341 S378 G379 H380 H400
BS02	rna	A	R2 V4 L20 D48 G426 G428	R2 V4 L20 D48 G426 G428
BS03	rna	A	R244 K280 N281 A286 G287	R244 K280 N281 A286 G287
BS04	ZN	A	D63 H64 D162 H400	D63 H64 D162 H400
BS05	ZN	A	H59 H61 H141	H59 H61 H141

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3iem, PDBe:3iem, PDBj:3iem
• PDBsum: 3iem
• UniProt: Q5SLP1|RNSE_THET8 Ribonuclease TTHA0252 (Gene Name=TTHA0252)