Structure of PDB 3i61 Chain A

Receptor sequence

>3i61A (length=509) Species: 4932 (Saccharomyces cerevisiae)

SKLIHVPKEDNSKEVTLDSLLEEGVLDKEIHKAITRMEFPGLTPVQQKTI
KPILSSEDHDVIARAKTGTGKTFAFLIPIFQHLINTKFDSQYMVKAVIVA
PTRDLALQIEAEVKKIHDMNYGLKKYACVSLVGGTDFRAAMNKMNKLRPN
IVIATPGRLIDVLEKYSNKFFRFVDYKVLDEADRLLEIGFRDDLETISGI
LNEKNSKSADNIKTLLFSATLDDKVQKLANNIMNKKECLFLDTVDKNEPE
AHERIDQSVVISEKFANSIFAAVEHIKKQIKERDSNYKAIIFAPTVKFTS
FLCSILKNEFKKDLPILEFHGKITQNKRTSLVKRFKKDESGILVCTDVGA
RGMDFPNVHEVLQIGVPSELANYIHRIGRTARSGKEGSSVLFICKDELPF
VRELEDAKNIVIAKQEKYEPSEEIKSEVLEAVTEEPEDISDIVISLISSY
RSCIKEYRFSERRILPEIASTYGVLLNDPQLKIPVSRRFLDKLGLSRSPI
GKAMFEIRD

3D structure

• PDB: 3i61 Structure of the Yeast DEAD box protein Mss116p reveals two wedges that crimp RNA
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P188 T189 R190 G220 G221 D223 F224 T242 G244 R245 D248 K252 F277 T382 V383 K384 H407 G408 R415 T433 D434 D528 S532 S539 F576	P101 T102 R103 G133 G134 D136 F137 T155 G157 R158 D161 K165 F190 T295 V296 K297 H320 G321 R328 T346 D347 D441 S445 S452 F489
BS02	ADP	A	F126 G128 T130 Q133 G155 G157 K158 T159 D441 R469 S470	F39 G41 T43 Q46 G68 G70 K71 T72 D354 R382 S383
BS03	BEF	A	T154 K158 R466 R469	T67 K71 R379 R382

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:3i61, PDBe:3i61, PDBj:3i61
• PDBsum: 3i61
• PubMed: 19748356
• UniProt: P15424|MS116_YEAST ATP-dependent RNA helicase MSS116, mitochondrial (Gene Name=MSS116)