Structure of PDB 3i5y Chain A

Receptor sequence

>3i5yA (length=509) Species: 4932 (Saccharomyces cerevisiae)

SKLIHVPKEDNSKEVTLDSLLEEGVLDKEIHKAITRMEFPGLTPVQQKTI
KPILSSEDHDVIARAKTGTGKTFAFLIPIFQHLINTKFDSQYMVKAVIVA
PTRDLALQIEAEVKKIHDMNYGLKKYACVSLVGGTDFRAAMNKMNKLRPN
IVIATPGRLIDVLEKYSNKFFRFVDYKVLDEADRLLEIGFRDDLETISGI
LNEKNSKSADNIKTLLFSATLDDKVQKLANNIMNKKECLFLDTVDKNEPE
AHERIDQSVVISEKFANSIFAAVEHIKKQIKERDSNYKAIIFAPTVKFTS
FLCSILKNEFKKDLPILEFHGKITQNKRTSLVKRFKKDESGILVCTDVGA
RGMDFPNVHEVLQIGVPSELANYIHRIGRTARSGKEGSSVLFICKDELPF
VRELEDAKNIVIAKQEKYEPSEEIKSEVLEAVTEEPEDISDIVISLISSY
RSCIKEYRFSERRILPEIASTYGVLLNDPQLKIPVSRRFLDKLGLSRSPI
GKAMFEIRD

3D structure

• PDB: 3i5y Structure of the Yeast DEAD box protein Mss116p reveals two wedges that crimp RNA
• Chain: A
• Resolution: 2.49 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P188 T189 R190 G220 G221 D223 F224 T242 G244 R245 D248 K252 F277 T382 V383 G408 R415 T433 D434 I531 S532 S536 F576	P101 T102 R103 G133 G134 D136 F137 T155 G157 R158 D161 K165 F190 T295 V296 G321 R328 T346 D347 I444 S445 S449 F489
BS02	ANP	A	F126 G128 T130 Q133 G155 G157 K158 T159 G439 D441 R466 R469	F39 G41 T43 Q46 G68 G70 K71 T72 G352 D354 R379 R382

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:3i5y, PDBe:3i5y, PDBj:3i5y
• PDBsum: 3i5y
• PubMed: 19748356
• UniProt: P15424|MS116_YEAST ATP-dependent RNA helicase MSS116, mitochondrial (Gene Name=MSS116)