Structure of PDB 3i12 Chain A

Receptor sequence
>3i12A (length=360) Species: 90371 (Salmonella enterica subsp. enterica serovar Typhimurium) [Search protein sequence]
AKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKAGQWHV
NDAENYLQNADDPAHIALRPSAISLAQVPGKHQHQLINAQNGQPLPTVDV
IFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLSSAACMDKDVAKRLLRDA
GLNIAPFITLTRTNRHAFSFAEVESRLGLPLFVKPANQGSSVGVSKVANE
AQYQQAVALAFEFDHKVVVEQGIKGREIECAVLGNDNPQASTCGEIVLNS
EFYDTKYIDDNGAQVVVPAQIPSEVNDKIRAIAIQAYQTLGCAGMARVDV
FLTADNEVVINEINTLPGFTNISMYPKLWQASGLGYTDLISRLIELALER
HTANNALKTT
3D structure
PDB3i12 The crystal structure of the D-alanyl-alanine synthetase A from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) E16 V19 Q22 H107 S191 Y260 R300 D302 E315 N317 G321 I325
Catalytic site (residue number reindexed from 1) E15 V18 Q21 H106 S190 Y257 R297 D299 E312 N314 G318 I322
Enzyme Commision number 6.3.2.4: D-alanine--D-alanine ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADP A K141 F183 K185 G190 S191 S192 E221 G223 I224 E228 L249 F304 E315 K140 F182 K184 G189 S190 S191 E220 G222 I223 E227 L248 F301 E312
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0005524 ATP binding
GO:0008716 D-alanine-D-alanine ligase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0008360 regulation of cell shape
GO:0009252 peptidoglycan biosynthetic process
GO:0071555 cell wall organization
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3i12, PDBe:3i12, PDBj:3i12
PDBsum3i12
PubMed
UniProtP0A1F0|DDLA_SALTY D-alanine--D-alanine ligase A (Gene Name=ddlA)

[Back to BioLiP]