Structure of PDB 3hxm Chain A

Receptor sequence

>3hxmA (length=637) Species: 262724 (Thermus thermophilus HB27)

GKTEVFLNRFALRPLNPEELRPWRLEVVLDPPPGREEVYPLLAQVARRAG
GVTVRMGDGLASWSPPEVLVLEGTLARMGQTYAYRLYPKGRRPLDPKDPG
ERSVLSALARRLLQERLRRLEGVWVEGLAVYRREHARGPGWRVLGGAVLD
LWVSDSGAFLLEVDPAYRILCEMSLEAWLAQGHPLPKRVRNAYDRRTWEL
LRLGEEDPSLLDYHASKGRVPVLTLEDLHEALSLPWEERRRRTREIASWI
GRRLGLGTPEAVRAQAYRLSIPKLMGRRAVSKPADALRVGFYRAQETALA
LLRLDGAQGWPEFLRRALLRAFGASGASLRLHTLHAHPSQGLAFREALRK
AKEEGVQAVLVLTPPMAWEDRNRLKALLLREGLPSQILNVPLREEERHRW
ENALLGLLAKAGLQVVALSGAYPAELAVGFDAGGRESFRFGGAACAVGGG
GHLLWTLPEAQAGERIPQEVVWDLLEETLWAFRRKAGRLPSRVLLLRDGR
VPQDEFALALEALAREGIAYDLVSVRKSGGGRVYPVQGRLADGLYVPLED
KTFLLLTVHRDFRGTPRPLKLVHEAGDTPLEALAHQIFHLTRLYPASGFA
FPRLPAPLHLADRLVKEVGRLGIRHLKEVDREKLFFV

3D structure

• PDB: 3hxm Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes.
• Chain: A
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 3.1.24.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	A170 Y171 R172 I173 Y226 H227 L281 M413 K422 Q433 I434 L435 N436 R446 N449 K457 R580 T613 R615 P650 R651 H657 R661	A166 Y167 R168 I169 Y213 H214 L234 M366 K375 Q386 I387 L388 N389 R399 N402 K410 R532 T565 R567 P602 R603 H609 R613
BS02	rna	A	K329 R444 H445	K282 R397 H398

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006260 DNA replication
• GO:0044355 clearance of foreign intracellular DNA

External links

• PDB: RCSB:3hxm, PDBe:3hxm, PDBj:3hxm
• PDBsum: 3hxm
• PubMed: 19812667
• UniProt: Q746M7|AGO_THET2 Protein argonaute (Gene Name=ago)