Structure of PDB 3hx0 Chain A

Receptor sequence

>3hx0A (length=330) Species: 9606 (Homo sapiens)

SQKATNHNLHITEKLEVLAKAYSVQGDKWRAAGYAKAINALKSFHKPVTS
YQEACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAG
TKTAQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEI
EQTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFS
RLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVP
YSEFACALLYFTGSAAFNASMRALAKTKGMSLSEHALSTAVVRNTHGAKV
GPGRVLPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 3hx0 Scrunching During DNA Repair Synthesis
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D182 D184 D245
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 Q471 K472 A514 R517 K521 L527 E529	W29 V217 Q226 K227 A269 R272 K276 L282 E284
BS02	dna	A	W342 G343 G345 K347 T348 D490 Y505	W97 G98 G100 K102 T103 D245 Y260
BS03	dna	A	Y267 W274 R275 G278 Y279 G304 G306 R308 M309 K312	Y22 W29 R30 G33 Y34 G59 G61 R63 M64 K67
BS04	dna	A	R389 Y418	R144 Y173
BS05	D3T	A	R386 G416 S417 R420 G426 D427 D429 Y505 G508 A510 N513	R141 G171 S172 R175 G181 D182 D184 Y260 G263 A265 N268
BS06	MG	A	D427 D429	D182 D184

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3hx0, PDBe:3hx0, PDBj:3hx0
• PDBsum: 3hx0
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)