Structure of PDB 3hwt Chain A

Receptor sequence

>3hwtA (length=319) Species: 9606 (Homo sapiens)

TEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACSIPGIG
KRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQG
FRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQAF
NSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEGF
LTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYF
TGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGAKVGPGRVLPTPTE
KDVFRLLGLPYREPAERDW

3D structure

• PDB: 3hwt Scrunching during DNA repair synthesis
• Chain: A
• Resolution: 1.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D171 D173 D234
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 L277 V462 S463 Q464 Q471 K472 H511 R514 R517 K521 L527 S528 E529 H530 H541	W18 L21 V206 S207 Q208 Q215 K216 H255 R258 R261 K265 L271 S272 E273 H274 H285
BS02	dna	A	W342 G343 G345 K347 T348 L474 D490 Y505	W86 G87 G89 K91 T92 L218 D234 Y249
BS03	dna	A	W274 R275 Y279 P303 G304 I305 G306 R308 M309	W18 R19 Y23 P47 G48 I49 G50 R52 M53
BS04	D3T	A	R386 S417 R420 G426 D427 D429 Y505 A510 N513	R130 S161 R164 G170 D171 D173 Y249 A254 N257
BS05	MG	A	D427 D429	D171 D173

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3hwt, PDBe:3hwt, PDBj:3hwt
• PDBsum: 3hwt
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)