Structure of PDB 3hw8 Chain A

Receptor sequence

>3hw8A (length=327) Species: 9606 (Homo sapiens)

ATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQE
ACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKT
AQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQT
VQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLL
DSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSE
FACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGAKVGPG
RVLPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 3hw8 Scrunching Durning DNA Repair Synthesis
• Chain: A
• Resolution: 1.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D179 D181 D242
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 L277 V462 S463 Q464 Q471 K472 H511 R514 R517 A518 K521 L527 S528 E529 H530 K544	W26 L29 V214 S215 Q216 Q223 K224 H263 R266 R269 A270 K273 L279 S280 E281 H282 K296
BS02	dna	A	W342 G343 G345 K347 T348 D490 Y505	W94 G95 G97 K99 T100 D242 Y257
BS03	dna	A	Y267 W274 R275 G278 Y279 P303 G304 I305 G306 R308 M309	Y19 W26 R27 G30 Y31 P55 G56 I57 G58 R60 M61
BS04	MG	A	D427 D429	D179 D181
BS05	D3T	A	R386 G416 S417 R420 G426 D427 D429 Y505 F506 A510 N513	R138 G168 S169 R172 G178 D179 D181 Y257 F258 A262 N265

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3hw8, PDBe:3hw8, PDBj:3hw8
• PDBsum: 3hw8
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)