Structure of PDB 3ho1 Chain A

Receptor sequence

>3ho1A (length=673) Species: 262724 (Thermus thermophilus HB27) [Search protein sequence]

NHLGKTEVFLNRFALRPLNPEELRPWRLEVVLDPPPGREEVYPLLAQVAR
RAGGVTVRMGDGLASWSPPEVLVLEGTLARMGQTYAYRLYPKGRRPLDPK
DPGERSVLSALARRLLQERLRRLEGVWVEGLAVYRREHARGPGWRVLGGA
VLDLWVSDSGAFLLEVDPAYRILCEMSLEAWLAQGHPLPKRVRNAYDRRT
WELLRLGEEDPKELSLLDYHASKGRLQGREGGRVAWVADPKKPIPHLTGL
LVPVLTLEDLHEEEGSLALSLPWEERRRRTREIASWIGRRLGLGTPEAVR
AQAYRLSIPKLMGRRAVSKPADALRVGFYRAQETALALLRLDGAQGWPEF
LRRALLRAFGASGASLRLHTLHAHPSQGLAFREALRKAKEEGVQAVLVLT
PPMAWEDRNRLKALLLREGLPSQILNVPLREEERHRWENALLGLLAKAGL
QVVALSGAYPAELAVGFDAGGRESFRFGGAACAVGGGHLLWTLPEAQAGE
RIPQEVVWDLLEETLWAFRRKAGRLPSRVLLLRNGRVPQDEFALALEALA
REGIAYDLVSVRKSGGGRVYPVQGRLADGLYVPLEDKTFLLLTVHRDFRG
TPRPLKLVHEAGDTPLEALAHQIFHLTRLYPASGFAFPRLPAPLHLADRL
VKEVGRLGIRHLKEVDREKLFFV

3D structure

PDB

3ho1 Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes.

Chain

Resolution

2.6 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.24.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	A170 Y171 R172 I173 R192 Y197 R200 Y226 H227 R232 I254 P255 L265 L267 L279 R286 M413 K422 Q433 I434 L435 N436 R446 N449 R580 T613 R615 P650 R651 H657 V685	A169 Y170 R171 I172 R191 Y196 R199 Y219 H220 R225 I244 P245 L255 L257 L269 R276 M403 K412 Q423 I424 L425 N426 R436 N439 R568 T601 R603 P638 R639 H645 V673
BS02	rna	A	L267 H445 N546 R548 R574 K575 S576 G577 R611 F647	L257 H435 N534 R536 R562 K563 S564 G565 R599 F635

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0004520	DNA endonuclease activity
GO:0030145	manganese ion binding
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0044355	clearance of foreign intracellular DNA

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Molecular Function

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Biological Process

External links

PDB	RCSB:3ho1, PDBe:3ho1, PDBj:3ho1
PDBsum	3ho1
PubMed	19812667
UniProt	Q746M7\|AGO_THET2 Protein argonaute (Gene Name=ago)

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