Structure of PDB 3hjr Chain A

Receptor sequence

>3hjrA (length=593) Species: 646 (Aeromonas sobria)

GCAPLTGKESGMDIGRSSTERCLPGANPLQDQQWYLLNSGQDGFSARGGI
AGNDLNLWWAHRTGVLGQGVNVAVVDDGLAIAHPDLADNVRPGSKNVVTG
SDDPTPTDPDTAHGTSVSGIIAAVDNAIGTKGIAPRAQLQGFNLLDDNSQ
QLQKDWLYALGDSNASRDNRVFNQSYGMSVVDPRSANSLDQSQLDRLFEQ
QTLKAQGAAYIKAAGNGFNKIAAGGYVLNRTGNGPKLPFENSNLDPSNSN
FWNLVVSALNADGVRSSYSSVGSNIFLSATGGEYGTDTPAMVTTDLPGCD
MGYNRTDDPSTNRLHGNSQLDASCDYNGVMNGTSSATPSTSGAMALLMSA
YPDLSVRDLRDLLARSATRVDAKHQPVMVSYTSSTGKVRDVKGLEGWERN
AAGMWFSPTYGFGLIDVNKALELAANHQPLPPLVQLPWQKINVTGSAAAI
ADVGNSPTSSTTRIATPLTVEAVQVMVSLDHQRLPDLLIELVSPAGTRSI
LLSPFNSLVGQSLDQQQLGFVRTKGLRDMRMLSNKFYGESAQGTWRLEVT
DVANGTRQVSLLNRETRERTTLTERNNRQPGKLISWSLRVLGH

3D structure

• PDB: 3hjr Structural basis for the kexin-like serine protease from Aeromonas sobria as a sepsis-causing factor
• Chain: A
• Resolution: 1.65 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D78 H115 N218 S336
• Catalytic site (residue number reindexed from 1): D76 H113 N216 S334
• Enzyme Commision number: 3.4.21.121: Lys-Lys/Arg-Xaa endopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	N29 D87 V126 N128 I130 T132	N27 D85 V124 N126 I128 T130
BS02	CA	A	D454 D488 D553 A555 N578 N579	D452 D486 D551 A553 N576 N577
BS03	CA	A	D297 L298 G300 M303 N306 C326	D295 L296 G298 M301 N304 C324

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region
• GO:0042597 periplasmic space

External links

• PDB: RCSB:3hjr, PDBe:3hjr, PDBj:3hjr
• PDBsum: 3hjr
• PubMed: 19654332
• UniProt: Q9L5A4|ASP_AERSO Aeromonas extracellular serine protease (Gene Name=asp)