Structure of PDB 3hiw Chain A

Receptor sequence
>3hiwA (length=257) Species: 3572 (Saponaria officinalis) [Search protein sequence]
VIIYELNLQGTTKAQYSTFLKQLRDDIKDPNLHYGGTNLPVIKRPVGPPK
FLRVNLKASTGTVSLAVQRSNLYVAAYLAKNNNKQFRAYYFKGFQITTNQ
LNNLFPEATGVSNQQELGYGESYPQIQNAAGVTRQQAGLGIKKLAESMTK
VNGVARVEKDEALFLLIVVQMVGEAARFKYIENLVLNNFDTAKEVEPVPD
RVIILENNWGLLSRAAKTANNGVFQTPLVLTSYAVPGVEWRVTTVAEVEI
GIFLNVD
3D structure
PDB3hiw Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins.
ChainA
Resolution1.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) V74 E174 R177
Catalytic site (residue number reindexed from 1) V74 E174 R177
Enzyme Commision number 3.2.2.22: rRNA N-glycosylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 C2X A N71 Y73 V74 E121 S122 Y123 V169 E174 R177 E206 N207 G210 R214 Y233 L254 N71 Y73 V74 E121 S122 Y123 V169 E174 R177 E206 N207 G210 R214 Y233 L254 MOAD: Ki=3.9nM
PDBbind-CN: -logKd/Ki=8.06,Ki=8.7nM
Gene Ontology
Molecular Function
GO:0016787 hydrolase activity
GO:0030598 rRNA N-glycosylase activity
GO:0090729 toxin activity
Biological Process
GO:0006952 defense response
GO:0017148 negative regulation of translation
GO:0035821 modulation of process of another organism

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Molecular Function
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Biological Process
External links
PDB RCSB:3hiw, PDBe:3hiw, PDBj:3hiw
PDBsum3hiw
PubMed19920175
UniProtQ2QEH4

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