Structure of PDB 3hio Chain A

Receptor sequence
>3hioA (length=259) Species: 3988 (Ricinus communis) [Search protein sequence]
QYPIINFTTAGATVQSYTNFIRAVRGRLTTGADVRHEIPVLPNRVGLPIN
QRFILVELSNHAELSVTLALDVTNAYVVGYRAGNSAYFFHPDNQEDAEAI
THLFTDVQNRYTFAFGGNYDRLEQLAGNLRENIELGNGPLEEAISALYYY
STGGTQLPTLARSFIICIQMISEAARFQYIEGEMRTRIRYNRRSAPDPSV
ITLENSWGRLSTAIQESNQGAFASPIQLQRRNGSKFSVYDVSILIPIIAL
MVYRCAPPP
3D structure
PDB3hio Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins.
ChainA
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) V81 E177 R180
Catalytic site (residue number reindexed from 1) V77 E173 R176
Enzyme Commision number 3.2.2.22: rRNA N-glycosylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 C2X A D75 N78 Y80 V81 D96 D100 G121 Y123 I172 R180 W211 G212 R213 R258 D71 N74 Y76 V77 D92 D96 G117 Y119 I168 R176 W207 G208 R209 R254 PDBbind-CN: -logKd/Ki=6.52,Ki=300nM
Gene Ontology
Molecular Function
GO:0030598 rRNA N-glycosylase activity
Biological Process
GO:0017148 negative regulation of translation

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Molecular Function
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Biological Process
External links
PDB RCSB:3hio, PDBe:3hio, PDBj:3hio
PDBsum3hio
PubMed19920175
UniProtP02879|RICI_RICCO Ricin

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